转铁蛋白受体在IgA肾病肾组织中的表达  

CD71 expression in renal tissue of IgA nephropathy

作　　者：张爱平[1] 王自良[2] 王艳侠[1] 丁尧海[1] 张磊[1] 姜晓宇[1] 石书梅[1] 李宏栋[1] 李震[1] 

机构地区：[1]济南军区总医院肾内科,250031 [2]山东莱芜市人民医院肾内科

出　　处：《中华肾脏病杂志》2007年第10期631-634,共4页Chinese Journal of Nephrology

摘　　要：目的阐明转铁蛋白受体（cD71、TfR）在IgA肾病（IgAN）肾组织中表达的分布特点及其与IgA表达的关系,探讨其在IgAN免疫发病机制中的作用。方法120例肾活检患者根据临床表现和肾活检病理诊断分为原发性IgAN组（原发组）44例、非IgAN性IgA沉积组（继发组）38例、无IgA沉积肾病组（肾病组）38例。用免疫荧光双套色法标记的抗体,在激光共聚焦荧光显微镜下观察四甲基罗丹明（TRITC）标记的CD71和异硫氰荧光素（FITC）标记的IgA在肾组织上的表达。结果CD71的表达强度与IgA的沉积程度相一致。CD71与IgA在原发组肾小球上高表达;在继发组低表达;在对照组微弱表达或不表达。激光共聚焦荧光显微镜下观察到CD71表达与IgA表达呈现共位现象。结论CD71在IgAN患者肾小球呈现高表达,其表达与IgA分子呈现共位状态,提示CD71可能参与了IgAN免疫发病过程。Objective To study the molecular pathologic features of CD71 and correlation between IgA deposits and CD71 expression in IgA nephropathy （IgAN）,and to investigate the role of CD71 in pathogenesis of IgAN. Methods According to clinical manifestations and pathological diagnosis, renal tissue samples of 120 cases were divided into IgA deposit nephritis （the primary group） 44 cases, non-IgAN with IgA deposit （the secondary group） 38 cases and no IgA deposit nephritis （the control group ） 38 cases. Immunofluorescence double-staining with TRITC-conjugated anti CD71 antibody and FITC-conjugated anti-human IgA antibody was performed to illustrate the expression of CD71 and IgA respectively under confocal microscopy. Results The expression of CD71 in the IgAN tissues was consistent with the accumulation of IgA. Significant differences of the expression of CD71 and IgA in glomeruli were found between primary and secondary group. The expression levels of CD71 and IgA were much higher in the primary group than those in secondary group. Moreover CD71 expression was not found in control group. IgA and CD71 were co-localized under confocal fluorescence microscopy. Condusion CD71 is highly expressed in glomeruli of primary IgAN and co-localized with IgA, which may play an important role in immunopathogenesis of IgAN.

关 键 词：肾小球肾炎 IgA 受体 转铁蛋白 

分 类 号：R686[医药卫生—骨科学]