地塞米松对嗅感觉神经元环核苷酸门控通道mRNA表达的影响  

Effects of steroid hormone on the expression of cyclic nucleotide-gated channels of olfactory receptor neurons

作　　者：张聪[1] 魏永祥[1] 苗旭涛[1] 刘小超[2] 邢飞虹[2] 赵红[1] 韩德民[1] 

机构地区：[1]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,北京100730 [2]首都医科大学附属北京同仁医院中心实验室,北京100730

出　　处：《中国耳鼻咽喉头颈外科》2007年第10期595-598,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：国家自然科学基金资助项目(30371534);耳鼻咽喉头颈科学教育部重点实验室(首都医科大学)

摘　　要：目的应用实时荧光定量逆转录聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR),观察地塞米松对大鼠嗅感觉神经元(olfactory receptor neurons,ORNs)环核苷酸门控通道(cyclic nucleotide-gated channels,CNG通道)A2亚基mRNA表达的影响。方法将40只Wistar大鼠随机分为4组(每组10只):24小时激素组、2周激素组及各自对照组。激素组大鼠腹腔注射地塞米松:24小时激素组按1mg/kg体重给药1次,2周激素组按0.2mg/天给药。各对照组均给予生理盐水腹腔注射。通过实时荧光定量RT-PCR方法,检测各组ORNs的CNG通道CNGA2亚基mRNA的表达量。结果24小时激素组和对照组CNG通道CNGA2亚基mRNA表达差异无显著性意义(P>0.05);2周激素组CNGA2亚基mRNA表达显著高于对照组(P<0.01)。结论地塞米松长期应用可以使ORNs的CNG通道CNGA2亚基mRNA表达上调,CNG通道的转录增强可能是糖皮质激素治疗嗅觉障碍的机制之一。OBJECTIVE To observe the effects of Dexamethasone on the expression of cyclic nucleotidegated channels （CNG channels） mRNA of olfactory receptor neurons （ORNs） by real-time quantitative reverse transcription-polymerase chain reaction（RT- PCR）. METHODS Forty Wistar rats were randomly divided into four groups： 24-hours Dexamethasone treated group and its control group; 2-weeks Dexamethasone treated group and its control group. Dexamethasone was injected i.p. （1 mg/kg for 24-hours group, 0.2 mg/d for 2-weeks group）. Control group rats were injected with the same volume of normal saline. Real-time quantitative RT-PCR was performed to evaluate mRNA production of CNGA2 subunits. RESULTS In Dexamethasone-injected rats, the upregulation of CNGA2 mRNA was observed in 2-weeks group（P 〈 0. 01）, but not in 24-hours group（P〉0.05）. CONCLUSION Steroid hormone could enhance the expression of CNG channel mRNA. Steroid hormone may thus contribute to the recovery of olfactory disorder partially, at least, through these effects on CNG channels.

关 键 词：糖皮质激素类 嗅觉受体神经元 环核苷酸调节蛋白激酶类 离子通道 

分 类 号：R765[医药卫生—耳鼻咽喉科]