采食高营养水平饲粮早期断奶羔羊禁饲状态下消化道pH及消化酶活性的研究  

The alimentary tract pH and digestive enzymes activity of early weaned fattening lambs in fasting with high nutritional level feed

作　　者：年芳[1] 杨子江[1] 郭江鹏[1] 马友记[1] 张军成[1] 王宏博[1] 王彩莲[1] 郝正里[1] 李发弟[1] 

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2007年第5期28-33,共6页Journal of Gansu Agricultural University

基　　金：甘肃省自然科学基金(3ZS061-A25-077);甘肃省教育厅高等学校研究生导师科研项目(0702-07)

摘　　要：研究了采食高营养水平饲粮早期断奶羔羊禁饲状态下其皱胃、胰腺和小肠pH及消化酶活性.按同质原则将24只1月龄断奶杂交羔羊（无角陶塞特♂×寒滩♀）分为3组,接受3种高营养饲粮,3种饲粮精粗比分别为97.64∶2.36、91.88∶8.12、86.14∶13.86,能量水平分别为14.54、13.97、13.36 MJ/kg,粗蛋白水平分别为25.30%、23.03%2、0.73%.饲喂65 d后,禁食24 h屠宰,测定羔羊皱胃、胰腺和小肠pH及消化酶活性.结果表明：高营养水平饲粮对羔羊胰腺、皱胃和小肠各段黏膜和内容物pH影响不大,对消化道各段分泌的主要消化酶活性无显著影响（P〉0.05）.十二指肠、空肠各段、回肠黏膜pH在6.55～6.86,十二指肠、空肠前段和空肠中段内容物pH为6.30～6.69,空肠后段、回肠内容物pH在7.22～7.73.胰蛋白酶活性在回肠最高,糜蛋白酶活性在各肠段无显著差异,α-淀粉酶活性在空肠中段和回肠最高,脂肪酶活性在空肠中段最高.The alimentary tract pH and digestive enzymes activity of early weaned fattening lambs with high nutritional feed when fasting 24 hours were studied. Twenty-four individuals of crossbreed lambs （Poll Dorest♂× Small-tail Han× Tan♀ ,50 days age） were divided into three groups at three high nutritional levels. The ratios of concentrate to roughage of three nutritional levels were 97.64 ＇ 2.36（A） ,91.88 ： 8.12（B）,and 86.14 ： 13.86（C）;crude protein rate were 25.30 %,23.03 % and 20.73%,energy levels were 14.54,13.97 MJ/kg and 13.36 MJ/kg respectively. The results showed that different high nutritional levels had no significant effect on pH of pancreas, abomasums and intestine mucous and content （P〉 0.05）. And different nutritional levels had no significant effect on activity of digestive enzymes of the fatte-ning lambs（P〉0.05）. The pH of mucosa was 6.55-6.86 in duodenum, jejunum and ileum. The pH of content was 6.30-6.69 in duodenum,anterior segment and middle piece of jejunum. The pH content was 7.22-7.73 in posterior segment of jejunum and ileum. Activity of trypsin was the highest in ileum, while there was no significant difference on activity of chymotrypsin intestine, Activity of a--amylase was the highest in middle piece of jejunum and ileum,while activity of lipase was the highest in middle piece of jejunum.

关 键 词：禁饲 营养水平 育肥羔羊 PH 消化酶 

分 类 号：S826.5[农业科学—畜牧学]