利用PCR技术快速检测玉米细菌性枯萎病菌(Pantoea stewartii subsp.stewartii)  

Application of PCR method in rapid identification of Pantoea stewartii subsp.stewartii

作　　者：吴兴海[1] 杨伟克[1] 邓明俊[1] 陈长法[1] 邵秀玲[1] 梁成珠[1] 封立平[1] 

机构地区：[1]山东出入境检验检疫局,山东青岛266002

出　　处：《甘肃农业大学学报》2007年第5期88-91,共4页Journal of Gansu Agricultural University

基　　金：国家质检总局植物病原检测专项基金(2005IK066)

摘　　要：根据玉米细菌性枯萎病菌(Pantoea stewartiisubsp.stewartii)ITS基因序列,设计并合成了特异性PCR检测引物,对玉米细菌性枯萎病菌和非玉米细菌性枯萎病菌的标准菌株进行了PCR扩增反应.结果显示,玉米细菌性枯萎病菌的PCR产物出现301 bp的特异性扩增条带,而非玉米细菌性枯萎病菌均未出现扩增条带;其PCR检测的灵敏度为612 pg/μL.因此PCR技术是一种特异、灵敏、快速检测玉米细菌性枯萎病菌的方法.A rapid identification method based on artii was established by using one pair of primers uni gene segments. A specific DNA amplicon was present molecular biology of Pantoea stewartii subsp, stewque to ITS（16 S-23 S rRNA interspacer region） in the standard strains,but absent in the non-stand strains,which showed that one pair of primers are a rapid molecular biological identification method of Pantoea stewartii subsp, stewartii. The sensitiv approximately 104,and the level of detection of ity th of this method was confirmed by serial dilutions while e primer was 612 pg/μL DNA solution.

关 键 词：PCR 玉米细菌性枯萎病菌 检测 

分 类 号：S435.131.49[农业科学—农业昆虫与害虫防治]