大鼠肝再生早期线粒体介导的caspase-3激活的机制  

Mechanism of mitochondria-mediated activation of apoptotic enzyme caspase-3 during early phase of liver regeneration in rats

作　　者：时多[1] 袁斌[1] 袁星[1] 蒋恒义[1] 孙学军[2] 缪明永[1] 焦炳华[1] 

机构地区：[1]第二军医大学基础部生物化学与分子生物学教研室,上海200433 [2]第二军医大学海军医学系潜水医学教研室

出　　处：《第二军医大学学报》2007年第10期1079-1081,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30570837).~~

摘　　要：目的:从线粒体介导的内源性途径分析大鼠肝再生早期肝细胞凋亡执行酶caspase-3激活的机制。方法:雄性SD大鼠35只,分成模型组15只(70%肝切除制作肝再生模型)、假手术组15只和正常对照组5只。模型组和假手术组根据时间分为术后3、6和24 h三个亚组,每个亚组5只。取各组大鼠肝组织,制备肝匀浆液。荧光法测肝组织凋亡酶caspase-3和caspase-9的活性;酶联免疫分析法测谷胱甘肽过氧化酶(GSH-Px)活性;硝酸还原法测NO含量。结果:与假手术组相比,模型组凋亡酶caspase-3和caspase-9的活性在肝再生早期(术后3、6 h组)显著增高(P<0.01);GSH-Px水平显著降低,NO分泌量显著增高(P<0.01)。结论:肝再生早期,肝组织处于氧化应激状态,线粒体介导的内源性途径参与凋亡执行酶的激活。Objective： To analyze the activation mechanism of hepatic apoptosis-related enzyme caspase-3 during the early phase of liver regeneration in rats from the perspective of mitochondria-mediated endogenous signal pathway. Methods： Thirtyfive male SD rats were randomly divided into 3 groups： model group [70% partial hepatectomy （PH）, n= 15], sham-operated group （n= 15）, and control group （n = 5）. The former 2 groups were further divided into 3 subgroups according to time after operation （3 h,6 h,and 24 h after operation,5 animals in each group）. The animals in all groups were sacrificed and the liver homogenates were prepared. Using the fluorescence method, we determined the activities of hepatic caspase-3 and caspase-9 at 3 different time points after operation. The activity of glutathione peroxidase （GSH-Px） and the content of NO were examined by enzyme immunoassay and nitrate reductase assay,respectively. Results： The activities of hepatic caspase-3 and 9 both increased significantly compared with those of sham-operated group （P〈0.01） at 3 h and 6 h after operation; the level of GSH-Px was significantly decreased and the content of NO was significantly increased compared with those of the sham-operated group （P〈 0.01）. Conclusion： Hepatic tissue is under oxidative stress during the early phase of liver regeneration,and the mitochondria-mediated endogenous signal pathway is involved in the activation of caspase-3.

关 键 词：肝再生 细胞凋亡 线粒体 caspase-3 CASPASE-9 氧化性应激 

分 类 号：R657.3[医药卫生—外科学] R349.31[医药卫生—临床医学]