AT1R基因多态性与肺原性心脏病的相关性  

Study of relationship between AT1R gene polymorphisms and chronic pulmonary heart disease

作　　者：郑红霞[1] 

机构地区：[1]济南军区青岛第二疗养院检验科,山东青岛266071

出　　处：《实用医药杂志》2007年第6期715-717,共3页Practical Journal of Medicine & Pharmacy

摘　　要：目的通过对肺心病患者AT1R基因多态性的检测,从分子水平探讨肺原性心脏病(肺心病)的发病机理,寻找肺心病发病的相关致病基因。方法应用多聚酶链反应(PCR)技术,检测40名正常人和60例肺心病患者AT1R、ACE的基因型和等位基因频率。结果①正常对照组AT1R基因型分布为AA型85.0%,AC型15.0%;肺心病组AT1R基因型分布为AA型70.3%,AC型29.7%;统计学分析两组间基因型构成、A和C等位基因频率均无显著性差异;②女性肺心病患者AT1R基因型分布AA型70.4%,AC型29.6%,无CC型,等位基因频率A型为85.2%,C型为14.8%;男性肺心病患者AT1R基因型分布为AA型69.7%,AC型30.3%,无CC型,等位基因频率A型为84.8%,C型为15.2%;统计学分析两组间基因型构成、等位基因频率无显著性差异;③在肺心病伴有肺栓塞组的19例患者AT1R基因AA型、AC型和CC型分别为84.2%、15.8%和0,等位基因频率A型为92.1%,C型为7.9%;统计学分析肺心病伴肺栓塞组与正常对照组基因型构成、等位基因频率比较无显著性差异。结论①AT1R A1166C多态性与肺心病发生以及肺心病伴肺栓塞无明显相关性;②肺心病组和AA基因型患者组与正常对照组平均红细胞压积无明显相关性。Objective To investigate the association between ATIR gene polymorphisms and pulmonary heart disease （CPHD） in view of molecular level.Methods The ACE and AT1R genotype in the control group （40 subjects）and the study group （60 patients） was detected by the polymerase chain reaction （PCR）, then allelic-gene frequeucy were calculated.Results 1）In the control group,the distribution of the AA,AC genotypes of the ATIR gene is 85.0%,15.0%.In the study group, the distribution of the AA,AC genotypes was 70.3%,29.7% respectively;There was also no difference of ATIR genetype and the allele frequency between the control group and the study group.2）In the female subgroup of CPHD, the distribution of the AA,AC and CC genotypes of the ATIR gene was 70.4%, 29.6% and 0 respectively; In the male subgroup, the distribution of the AA,AC and CC genotypes of the ATIR gene was 69.7%, 30.3% and 0 respectively; These percentages in female subgroup significantly differed from the male subgroup, A/C allele frequency in female subgroup did not significantly differ from the male subgroup.3） In 19 subjects of patients of CPHD accompanying with pulmonary embolism,the distribution of the AA, AC and CC genotypes of the ATIR gene was 84.2%,15.8% and 0 respectively.A/C allele frequency was 92.1% and 7.9%. The percentages of ATIR genotypes was not significantly different between control group and subgroup, the A and C allele frequencies were significant association between the two groups. Conclusion 1） There was no association between ATIR gene polymorphism and CPDH,also pulmonary embolism.2）AA genotype is not association with the mean Hct of control group.

关 键 词：AT1R基因 基因多态性 肺原性心脏病 

分 类 号：R541.5[医药卫生—心血管疾病]