脂多糖结合蛋白与CD14基因多态性分布及其意义初步探讨  

The distribution and potential significance of lipopolysaccharide binding protein and CD14 gene polymorphism

作　　者：蔺静[1] 姚咏明[1] 黄志红[1] 于燕[1] 柴家科[1] 于勇[1] 

机构地区：[1]解放军总医院第一附属医院全军烧伤研究所,北京市100037

出　　处：《中华老年多器官疾病杂志》2007年第5期320-324,共5页Chinese Journal of Multiple Organ Diseases in the Elderly

基　　金：国家重点基础研究发展规划项目(2005CB522602);国家杰出青年科学基金资助项目(30125020);北京市科技计划重大项目(H020920020530);首都医学发展基金重点项目(2003-2023)

摘　　要：目的通过探讨脂多糖结合蛋白(LBP)Leu436→Phe基因多态性对CD14合成与释放的影响以及CD14 C-159T对LBP蛋白浓度的调节效应,阐明这两种基因多态性的可能作用机制。方法研究对象为118例健康献血员及26例烧伤面积>30%的患者,采用限制性片段长度多态性分析LBPLeu436→Phe及CD14 C-159T基因多态性,同时检测CD14 mRNA表达、可溶性CD14(sCD14)及LBP浓度。结果体外全血培养实验中,脂多糖刺激与否,LBPLeu436→Phe对CD14 mRNA表达、sCD14水平均无明显影响(P>0.05),CD14-159位点基因型也与LBP浓度无关(P>0.05)。而对烧伤面积>30%的患者研究显示,CD14-159位点TT基因型患者LBP浓度于伤后第3、21天明显高于CC基因型(P<0.05)。结论CD14 C-159T基因多态性可能通过间接调节机制影响LBP产生,并与严重烧伤患者预后不良有关。Objective To determine the possible mechanism of lipopolysaccharide binding protein （LBP） Leu436→Phe and CD14 C-159T genetic polymorphism in terms of the influence of LBP Leu436→Phe genetic polymorphism on lipopolysaccharide receptor CD14 gene expression as well as protein release, and the potential regulating eftect of CD14 C-159T on LBP levels. Methods The study group consisted of 118 healthy blood donors and 26 patients with burns covering more than 30% of total body surface area. The LBP and CD14 genetic polymorphisms were detected by restrictionragment length polymorphism analysis. Meanwhile, the CD14 mRNA expression, soluble CDI4 （sCD14） and I.BP levels were determined with ELISA methods. Results LBP Leu436→Phe had no marked influence on CD14 mRNA expression as well as CD14 levels （all P〉0.05）, and the genotype of CD14 at position -159 site was not related to LBP levels in in vitro whole blood culture with or without lipopolysaccharide stimulation （100 μg/L for 12 h）. However, in patients with burns covering more than 30% of total body surface area, TT genotype showed significantly higher LBP levels than CC homozygotes on days 3 and 21 post burn （P〈0.05）. Conclusion CD14C-159T polymorphism might affect LBP production indirectly through the action of cytokines, which appears to be associated with the poor outcome of patients with major burns.

关 键 词：烧伤 脂多糖类 抗原 CD14 基因多态性 

分 类 号：R392.11[医药卫生—免疫学]