黄芪甲苷对异丙肾上腺素所致小鼠心肌肥厚的保护作用  

Protective effects of astragaloside Ⅳ on isoproterenol-induced cardiac hypertrophy in mice

作　　者：许晓乐[1] 季晖[1] 谷舒怡[1] 黄秋菊[1] 陈艳萍[1] 

机构地区：[1]中国药科大学药理学教研室,南京210009

出　　处：《中国药科大学学报》2007年第5期451-455,共5页Journal of China Pharmaceutical University

基　　金：中国药科大学药学医学基础实验教学中心开放课题

摘　　要：目的:考察黄芪甲苷对异丙肾上腺素所致小鼠心肌肥厚的保护作用并探讨其机制。方法:皮下注射异丙肾上腺素致小鼠心肌肥厚模型,以黄芪甲苷(40,80mg/kg)和阳性对照普萘洛尔(40mg/kg)灌胃给药,测量小鼠心脏重量指数和心肌羟脯氨酸(Hyp)含量,血清乳酸脱氢酶(LDH)、肌酸激酶(CK)活力,心肌组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量;RT-PCR法检测心肌肌浆网钙泵(SERCA2a)和受磷蛋白(PLB)的mRNA表达。结果:与正常对照组相比,异丙肾上腺素组心脏重量指数、心肌Hyp含量、血清LDH和CK水平显著升高,心肌SOD活性下降,心肌MDA含量增加,SERCA2amRNA表达显著减少,PLBmRNA表达无明显变化。与异丙肾上腺素组相比,黄芪甲苷降低心脏重量指数和心肌Hyp含量,降低血清LDH和CK水平,增加心肌SOD活性,降低心肌MDA含量,上调SERCA2amRNA表达。结论:黄芪甲苷对异丙肾上腺素诱导的小鼠心肌肥厚具有保护作用,其机制可能与清除氧自由基,增加SERCA2amRNA表达有关。Aim：To study the effects and the mechanism of astragaloside Ⅳ on isoproterenol-induced cardiac hypertrophy in mice. Methods：Myocardial injury in mice was induced by subcutaneous injection of isoproterenol. Astragaloside Ⅳ （40,80 mg/kg） and propranolol （40 mg/kg, positive drug） were then administered to mice via garage. The heart weight indexes, the content of hydroxyproline （Hyp） in myocardium, serum lactate dehydrogenase （LDH） and creatine kinase （CK）, the malondialdehyde （MDA） and activity of superoxide dismutase （SOD） in myocardium were measured. Expressions of sarcoplasmic reticulum Ca^2＋ -ATPase （SERCA2a） mRNA and phospholamban （PLB） mRNA in myocardium were detected by RT-PCR. Results： Compared with the control, the heart weight indexes and myocardial Hyp content as well as serum LDH and CK were markedly increased, myocardial SOD activity was decreased and MDA content was increased in isoproterenol-treated mice. RT-PCR analysis showed that the level of SERCA2a mRNA expression was decreased while the level of PLB mRNA expression was not significantly changed in isoproterenol-alone group compared with the control group. Treatment with astragaloside Ⅳ reduced heart weight indexes, myocardial Hyp content, serum LDH and CK, increased myocardial SOD activity and decreased MDA content compared with the isoproterenol-treated group. In addition, astragaloside Ⅳ attenuated the depression of SERCA2a mRNA level compared with isoproterenol-treated mice. Conclusion： Astragaloside Ⅳ possesses cardioprotective effects against isoproterenolinduced cardiac hypertrophy in mice. This may be related to the reduction in the oxidative stress and increase of ERCA2a mRNA level in myocardium.

关 键 词：黄芪甲苷 异丙肾上腺素 心肌肥厚 肌浆网钙泵 脂质过氧化 

分 类 号：R285.5[医药卫生—中药学]