人Kv1．5通道基因转染人胚胎肾细胞的研究  

Study of transfecting pcDNAHKv1.5 into human embryonic kidney cells

作　　者：刘坤[1] 高翔[1] 田苗[1] 刘金平[1] 张昌伟[1] 水志刚[1] 陈志坚[1] 廖玉华[1] 

机构地区：[1]华中科技大学附属协和医院心血管病研究所,武汉430022

出　　处：《公共卫生与预防医学》2007年第5期6-8,共3页Journal of Public Health and Preventive Medicine

基　　金：国家自然科学基金(30570729)

摘　　要：目的建立表达人Kv1.5通道的细胞模型。方法采用Lipofactamine 2000脂质体将人Kv1.5通道基因转染人胚胎肾细胞(HEK293),通过荧光显微镜和全细胞膜片钳技术观测人Kv1.5通道基因的表达。结果pcDNAHKv1.5真核表达载体转染HEK293细胞2d后,荧光显微镜可观察到人Kv1.5通道表达;3d后全细胞膜片钳技术记录到人Kv1.5通道基因编码的通道电流。结论pcDNAHKv1.5真核表达载体转染HEK293细胞为人Kv1.5通道的研究提供了良好的真核细胞表达系统和细胞模型。Objective To establish a cell model for the expression of human Kv1. 5. Methods pcDNAHKv1. 5 was transfected with lipofectamine 2 000 into human embryonic kidney cells （HEK293）. The expression of human Kv1. 5 gene was detected by fluorescence microscope and whole cell patch clamp. Results The expression of human Kv1. 5 gene was observed by fluorescence microscope two days after the transfection and the encoded currents were recorded by whole cell patch clamp 3 days after the transfection. Conclusion It provides a good eukaryocytic expression system and cell model for the study of human Kvl. 5 to transfect pcDNAHKv1. 5 into HEK293 cells.

关 键 词：人Kv1.5通道 人胚胎肾细胞 转染 

分 类 号：R965.1[医药卫生—药理学]