噬菌体生物扩增法检测痰结核分枝杆菌的临床研究  

Clinical study of the application of phage amplified biologically assay in the detection of mycobacterium tuberculosis in sputum

作　　者：于秀丽[1] 王秋月[1] 李艳玲[1] 邢勇[2] 侯刚[1] 康健[1] 

机构地区：[1]中国医科大学附属第一医院呼吸疾病研究所,辽宁沈阳110001 [2]沈阳市胸科医院,辽宁沈阳110044

出　　处：《中国实用内科杂志》2007年第21期1697-1699,共3页Chinese Journal of Practical Internal Medicine

摘　　要：目的评价噬菌体生物扩增(PhaB)法检测痰中结核分枝杆菌的应用价值。方法分别应用PhaB法、直接涂片法、集菌涂片法和BACTECMGIT960快速培养法检测2005年8月至10月沈阳市胸科医院53例疑诊肺结核患者痰标本中结核分枝杆菌,并对检测结果进行比较。结果PhaB法、直接涂片法、集菌涂片法、BACTECMGIT960快速培养法检出阳性标本数分别为24,11,17和22份;以培养结果为评价标准,PhaB法的敏感性、特异性、阳性预测值、阴性预测值分别为86.4%,83.9%,79.2%和89.7%;集菌涂片法和PhaB法联合检测的敏感性为90.9%,特异性为83.9%,阳性、阴性预测值分别为80.0%和92.9%。结论PhaB法检测痰中结核菌操作简便,具有较高的敏感性和特异性,可作为临床结核分枝杆菌的快速检测方法;PhaB法联合集菌涂片法进行检测可进一步提高敏感性,减少漏诊率。Objective To assess the application of the phage amplified biologically assay （PhaB） in the detection of the mycobacterium tuberculosis （TB） in sputum. Methods To determine the TB patients in the 53 suspected patients we detected 53 sputum samples by PhaB method, BACTEC MGIT960 rapid culture method, and conventional methods （ direct auramine smear microscopy and concentrated auramine smear microscopy） individually. Results TB positive samples detected by PhaB, BACTEC MGIT960 rapid culture, direct auramine smear microscopy and concentrated auramine smear mi croscopy were 24,22,11 and 17 respectively. Thus the sensitivity, specificity,positive predictive value and negative predictive value were 86.4% ,83.9% ,79. 2% and 89.7% ,when the PhaB is combined with concentrated auramine smear microscopy,the sensitivity, specificity, positive predictive value and negative predictive value were increased up to 90. 9% ,83.9% ,80. 8% and 92. 9%. Conclusion PhaB method is a simple and rapid method to detect the Mycobacterium Tuberculosis in sputum with a relatively higher sensitivity and specificity than the conventional methods. When combined with concentrated auramine smear microscopy, PhaB method is even more sensitive. Thus the PhaB method is accessible and applicable in the clinical use of the TB detection.

关 键 词：分枝杆菌 结核 分枝杆菌噬菌体 

分 类 号：R5[医药卫生—内科学]