冰浴时间对氯化钙转化法转化效率的影响  

Effects of Freezing Time on the Transforming Efficiency of CaCl_2 Method

作　　者：张卫林[1] 赵晶[1] 李芬[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《安徽农业科学》2007年第30期9571-9571,共1页Journal of Anhui Agricultural Sciences

基　　金：河南师范大学青年科学基金(20040012);国家自然科学基金(30600343)

摘　　要：确定大肠杆菌DH5α氯化钙转化法时感受态细胞与质粒混合物的最佳冰浴时间。方法:在保证其他条件一致的情况下,设置大肠杆菌感受态细胞和质粒混合物的冰浴时间梯度并建立阴性对照进行研究。结果:冰浴20 min时转化效率最高。结论:与其他冰浴时间相比,冰浴20 min时的转化效率最高,实验重复性好,成本低廉,而且操作简便,值得推广使用。The aim of the research was to confirm the best freezing time of the mixture of competented cell and plasmid in the E.coli DH5α CaCl2 transforming method.The method was to set up a freezing time ladder of the mixture of competented cell and plasmid along with a negative sample to research under the same condition of other aspects;The results revealed that the transforming efficiency was the highest at the freezing time of 20 minutes;Compared with the efficiency of other freezing times,the 20 minutes freezing efficiency was the highest and the assay can be repeated easily,so it deserved using widely.

关 键 词：冰浴 感受态细胞 转化效率 

分 类 号：O64[理学—物理化学]