禽传染性支气管炎病毒分离毒株的血清型鉴定与免疫防制研究  

Studies on Serotype Identification and Immune Control of Isolated Strains of Avian Infectious Bronchitis Virus

作　　者：吕化广[1,2] 杨奇伟[1,2] 张洪勇[1,2] 武志强 陈伟 付朝阳[1,2] 

机构地区：[1]沈阳农业大学畜牧兽医学院 [2]中国农业科学院哈尔滨兽医研究所

出　　处：《中国兽医杂志》1997年第8期7-10,共4页Chinese Journal of Veterinary Medicine

摘　　要：应用基于单克隆抗体的间接免疫荧光实验（Ｍａｂ－ＩＦＡ）和病毒血清中和实验，分别对１１个禽传染性支气管炎病毒（ＩＢＶ）分离毒株进行血清型鉴定。其中３株为Ｍａｓ型，２株为Ａｒｋ型，２株为Ａｒｋ／Ｍａｓｓ混合型，其余４株为不可分类型或未知型。将４株不可分类型毒株混合制成ＩＢＶ分离毒株油乳灭能苗。经ＳＰＦ鸡免疫攻毒实验证明，这种不可分类型毒株灭能苗与Ｈ１２０弱毒苗合用能够有效抵抗各不可分类型毒株的攻毒感染，保护率为８４％～１００％。另外，ＳＰＦ实验鸡经Ｈ１２０和Ａｒｋ二联苗免疫后对Ａｒｋ型分离毒株和Ａｒｋ／Ｍａｓ混合型分离毒株的保护率为７５％～８５％。但是，虽经Ｈ１２０弱毒苗免疫ＳＰＦ鸡则不能抵抗ＩＢＶ不可分类型毒株。Eleven field isolates of infectious bronchitis virus (IBV)reported in a previous study were serotyped by using a combination of a IBV monoclonal antibodies (Mabs) based indirect fluorescent antibody (IFA) test and by virus neutralization (VN) test in chicken trachealring organ cultures (TROC) with antisera to IBV reference strains.Of 11 isolates serotyped,3 were Massachusetts (Mass),2 were Arkansas (Ark),2 were a combination of Mass and 4 were untypeable.This is the first report of the presence of Ark and/or Arkrelated serotypes and other untypeable IBV isolates in China.An inactivatcd oil emulsion vaccinc made from a pool of 4 untypeable IBV isolates was developed in our laboratory for experimental and field use.Vaccine challenge studies demonstrated that the inactivated vaccine provided 84～100% protection (based on virus isolation from tracheal swabs at days post challenge) in SPF chickens against challenges with the untypeable isolates.IBV H120 and Ark vaccines gave 75～85% protection against challenges with Ark and Ark/Mass isolates.

关 键 词：禽病 IBV 分离毒株 单克隆抗体 血清型 Mab-IFA 

分 类 号：S852.65[农业科学—基础兽医学]