微梁传感研究大分子构象转变  

Investigation on Conformation Transition of Macromolecules using Microcantilever Sensing

作　　者：李凯[1] 张青川[1] 刘红[2] 

机构地区：[1]中国科学技术大学中科院材料力学行为和设计重点实验室,合肥230027 [2]中国科学技术大学化学物理系,合肥230026

出　　处：《实验力学》2007年第3期395-400,共6页Journal of Experimental Mechanics

基　　金：国家重点基础研究发展计划(973计划;2006CB300404);国家自然科学重点基金(10232030);面上基金(10472112)

摘　　要：通过表面修饰技术将大分子连接到微悬臂梁的单侧表面上,调节周围的物理、化学环境使大分子的构象发生转变,并用光杠杆法检测微梁变形。实验结果显示,大分子在微梁表面的构象转变过程会引起微梁的表面应力发生变化,并使之产生纳米量级的端部位移变形。此外,对于不同的大分子,促使微梁表面应力发生变化的机制是各不相同的。分析显示,氢键作用、静电作用和疏水作用分别在PNIPAM分子、PAA分子和胰蛋白酶分子的构象转变和微梁表面应力变化过程中起主要作用。微梁传感用于大分子的构象转变检测,提取的是分子间相互作用力的信息,它为从微观上理解大分子构象转变问题提供了一种新的实验手段。Macromolecules were grafted on one surface of a technique. The deflections of the microcantilever induced macromolecules were detected using an opti＇cal lever method. surface stress of the microcantilever macromolecules, and as a consequence, macromolecules, the changes of surface analysis indicates that hydrogen bonding, roles in the conformation transition process changed during microcantilever by surface modification by the conformation transition of the The experimental result shows that the the conformation transition of the the microcantilever deflected. Morever, for different stress were originated from different mechanisms. The electrostatic and hydrophobic interactions play important of PNIPAM, PAA and trypsin, and the changes of surface stress. The most prominent advantage of this microcantilever sensing technique is that it could detect the interaction among macromolecules and thus provides a new experimental means for understanding the conformation transition process of macromolecules at a microscopic level.

关 键 词：微悬臂梁 表面应力 构象转变 PNIPAM PAA 胰蛋白酶 

分 类 号：O63[理学—高分子化学] O64[理学—化学]