探讨BCG初次免疫结核杆菌DNA疫苗加强免疫方案的效应  

Effect of the immune strategy based on BCG priming and Ag85A/GM-CSF DNA vaccine boosting in mice

作　　者：唐权[1] 窦骏[1] 赵枫姝[1] 禇莉莉 潘猛[1] 王永仿[1] 

机构地区：[1]东南大学基础医学院病原生物学与免疫学系,江苏南京210009

出　　处：《细胞与分子免疫学杂志》2007年第7期591-594,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：东南大学科研基金资助(92230021991)

摘　　要：目的:探讨BCG初次免疫(BCG-prime),结核杆菌共表达DNA疫苗加强免疫(DNA疫苗-boost)的策略对小鼠的免疫效果。方法:将BCG及结核杆菌重组DNA疫苗依次免疫小鼠,通过检测CTL和NK细胞的杀伤活性和特异性淋巴细胞增殖,以及小鼠血清抗体及细胞因子的水平,观测BCG-prime、共表达结核杆菌Ag85A/GM-CSFDNA疫苗boost策略对小鼠的免疫效果。结果:采用prime-boost免疫策略组的小鼠CTL的杀伤活性明显增强、特异性淋巴细胞明显增殖、IFN-γ的水平明显增高,NK细胞杀伤活性与对照组相比也有一定提高,但未超过BCG单独免疫效果。免疫小鼠血清特异性抗体的滴度超过单独DNA疫苗免疫组。结论:在采用BCG-prime-结核杆菌DNA疫苗boost免疫策略后,能增强对小鼠的免疫效应,尤其是Th1型细胞免疫反应增强明显,为进一步在动物体内进行保护性效应试验的研究提供了实验依据。AIM： To investigate immune effect in mice on the basis of BCG priming and DNA vaccine boosting, and to provide a new strategy for development of new type of anti-tuberculosis DNA vaccine further. METHODS： After mice were inoculated with BCG of 1 × 10^6 clone formation unit each three weeks, 100 μg of DNA vaccine was injected intramuscularly in mice two times at 3 week intervals. The proliferative responses of murine cytotoxic T lymphocytes （CTL）, natural killer （NK） and spleen cell to antigen 85A （Ag85A） were measured by M＇IF method respectively. The antibody titers and IFN-γ level from the immunized mice were detected by ELISA. RESULTS： The proliferative responses of CTL and spleen cell to Ag85A, as well as IFN-γ level in mice immunized with prime-boost strategy were significantly increased respectively compared with the control mice immunized with blank plasmid or BCG only. Although NK activity was a little higher in mice immunized with primeboost strategy than that of immunized with blank plasmid mice, it was still lower than that of mice immunized with BCG alone. The titer of the specific antibody against Ag85A in mice immunized with prime-boost strategy was also higher than that of mice immunized with DNA vaccine alone. CONCLUSION： The immune strategy of BCG-prime and Ag85A/GM-CSF DNA vaccine boost improve immune effect, especially the Thl cellular immune response increase obviously. This study provides the possibility of further research for investigating protective function in immunized mice challenged by Mycobactedum tuberculosis.

关 键 词：结核杆菌 抗原85A BCG DNA疫苗 prime-boost免疫策略 

分 类 号：R392.11[医药卫生—免疫学]