江苏省宜兴地区丙型肝炎病毒F蛋白抗体检测  

Detection of serum anti-F antibody in hepatitis C virus infected patients

作　　者：邓小昭[1] 蒋春梅[2] 许可[2] 王忠灿[1] 丁伟良 喻荣彬[2] 王洁[2] 吴超[4] 张云[1] 

机构地区：[1]南京军区军事医学研究所,210002 [2]南京医科大学公共卫生学院流行病与统计学系 [3]宜兴市第一人民医院 [4]南京大学医学院附属鼓楼医院

出　　处：《中华流行病学杂志》2007年第11期1116-1118,共3页Chinese Journal of Epidemiology

基　　金：江苏省高校自然科学研究指导性计划基金资助项目(03KJD330144);江苏省自然科学基金资助项目(BK2004011)

摘　　要：目的检测江苏省宜兴地区丙型肝炎(丙肝)患者血清丙肝病毒(HCV)-F抗体及其分布。方法利用基因重组获得的HCV-F/GST蛋白作为抗原,包被酶联反应板,ELISA间接法检测120例丙肝患者、15例乙肝患者、3例戊肝患者及10份正常人血清HCV-F抗体,结合丙肝患者的临床资料及疾病特征,统计分析HCV-F抗体的分布情况及与HCV感染的关系。结果120例丙肝患者血清HCV-F抗体阳性82例,阳性率68%,而15例乙肝、3例戊肝患者及10份正常人血清标本均未检出阳性;资料分析显示HCV-F抗体阳性率与HCV患者年龄、临床类型之间差异有统计学意义,51岁以上年龄组的HCV-F抗体阳性率是20～50岁组的6.675倍(95%CI:2.407～19.071),并且随着病程进展,阳性率也随之增高(OR=2.749,95%CI:1.470～5.141)。结论江苏省宜兴地区HCV感染者血清中存在HCV-F抗体,并可能与病程相关。Objective To assess the prevalence of serum anti-F in patients with hepatitis C virus （HCV） infection and the distribution of anti-F. Methods The recombinant protein （HCV-F/GST） was coated onto micro titer plates as antigen. Sera of 120 patients with hepatitis C virus infection, 15 patients with hepatitis B,3 patients with hepatitis E and 10 normal sera were tested by indirect ELISA for detecting anti-F. Results 82 samples out of the 120 （68%） HCV infected patients exhibited a positive anti-F reaction,showing significant difference from the controls with no HCV infection （P 〈 0.01 ）. Data from logistic analysis showed that the positive rate of anti-F was higher in patients over 50 year olds （OR = 6. 675,95 % CI ： 2. 407-19. 071 ）. Patients of midrange, severe phase and hepatic cirrhosis had higher rate than the others（OR =2.749,95% CI：1.470-5. 141） .Conclusion Prevalence and distribution of anti-F in Yixing hepatitis C patients was reported and which might be related to the progression of HCV infection.

关 键 词：丙型肝炎病毒 F蛋白 检测 

分 类 号：R446.6[医药卫生—诊断学]