牡蛎天然活性肽BPO-1诱导人胃腺癌BGC-823细胞凋亡的形态与超微结构观察  

Effects of oyster bioactive peptides BPO-1 on the morphology and ultrastructure of human gastric adenocarcinoma cell line BGC-823

作　　者：石松林[1] 李鹏[1] 李祺福[1] 梁盈[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《中国海洋药物》2007年第5期6-12,共7页Chinese Journal of Marine Drugs

基　　金：福建省科技计划重点资助项目(2003N0052)

摘　　要：目的观察BPO-1对人胃腺癌BGC-823细胞形态与超微结构的影响,以探索BPO-1对胃癌细胞的作用机制。方法采用酸抽提、凝胶柱层析等方法,从牡蛎体内分离提取到牡蛎天然活性多肽组分BPO-1,以细胞凋亡诱导物姜黄素和癌细胞分化诱导物HMBA为平行对照,用光镜、透射电镜观察BPO-1处理细胞的形态和超微结构变化。结果经处理后的BGC-823细胞体积缩小、染色质凝聚、线粒体空泡化、内质网腔扩大和出现凋亡小体等多种典型的细胞凋亡形态和超微结构特征。结论牡蛎天然活性肽BPO-1对人胃腺癌BGC-823细胞凋亡的诱导具有显著作用。Objective To study the effects of bioactive peptides from oyster on the morphology and ultrastructure changes of human gastric adenocarcinoma cell line BGC-823 and its mechanism. Methods BPO-1 was isolated from Ostrea cucullata by acid extraction and gel chromatography, and was used to treat the BGC-823 cells. Curcumin and HMBA were used as control. The morphology and ultrastructure of the cells were examined by light microscopy and transmission electron microscopy. Results After treatment with BPO-1, typical apoptosis characteristics of the morphology and ultrastructure could be observed on BGC-823 cells. Conclusion BPO-1 could effectively inhibit the proliferation of BGC-823 cells and induce BGC- 823 cells to apoptosis.

关 键 词：胃癌细胞 牡蛎天然活性肽 细胞凋亡 细胞形态与超微结构 

分 类 号：R931.711[医药卫生—生药学] R965[医药卫生—药学]