藻蓝蛋白对体外培养缺氧PC12细胞保护作用的最佳剂量  

Best protecting dosage of phycocyanin on PC12 cells after anoxia/re-oxygen in vitro

作　　者：张睿[1] 郭恒照[2] 刘吉东[3] 陈红兵[1] 段德麟[3] 郭云良[1] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266003 [2]山东省济宁医学院,山东济宁272100 [3]中国科学院海洋研究所,山东青岛266071

出　　处：《中国海洋药物》2007年第5期17-20,共4页Chinese Journal of Marine Drugs

基　　金：山东省科技攻关计划项目(2006GG2302019)

摘　　要：目的探讨藻蓝蛋白对体外培养缺氧PC12细胞保护作用的最佳剂量。方法将培养好的PC12细胞制成缺氧／复氧模型，应用藻蓝蛋白干预治疗。噻唑蓝（MTT）比色法检测PC12细胞的吸光度，观察细胞的活性及数量的变化；共聚焦显微镜测定Rhodamine123标记的PC12细胞内的荧光强度，观察线粒体膜电位的变化。结果缺氧／复氧后，PC12细胞的吸光度和细胞内的荧光强度均明显降低。藻蓝蛋白治疗后，各剂量组PC12细胞的吸光度明显升高，以5μg·mL^-1组最显著，P〈0．01；细胞内的荧光强度明显增强，以10ng·mL^-1组最显著，P〈0．01。结论藻蓝蛋白对缺氧／复氧后PCI2细胞保护作用的最佳剂量可能为5～10μg·mL^-1。Objective To study the best protecting dosage of phycocyanin on PC12 cells after anoxia/re-oxygen in vitro. Methods The anoxia/re-oxygen cell models were established with cultured PC12 cells and treated by phycocyanin. The absorbency of PC12 cells was determined by MTT method to observe the activity and number of PC12 cells after anoxia/re-oxygen. The fluorescent intensity in PC12 cells labeled by Rhodamine123 was measured with laser scanning confocal microscope to observe the mitochondrial membrane potential after anoxia/re-oxygen. Results After anoxia/re-oxygen, the absorbency and fluorescent intensity in PC12 cells were significantly lower than those in control group. After treatment with phycocyanin, the absorbency of PC12 cell in test groups was higher than that in model group, especially in 5μg · mL^-1 subgroup, P〈0.01; the fluorescent intensity in PC12 cells was also higher than that in model group, especially in 10μg · mL^-1 subgroup, P〈0.01. Conclusion The best protecting dosage of phycocyanin on PC12 cells after anoxia/re-oxygen in vitro might be 5-10μg · mL^-1.

关 键 词：缺氧/复氧 藻蓝蛋白 噻唑蓝 罗丹明123 线粒体膜电住 

分 类 号：R931.711[医药卫生—生药学] R965[医药卫生—药学]