RP-HPLC对金银花和连翘单煎、单煎后合并及混煎的分析  

RP-HPLC Analysis of single,merging,and boiled together Lonicera japonica and Forsythia suspense

作　　者：林丽美[1] 王智民[1] 王维皓[1] 李春[1] 曹伶俐[1] 张小光[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2007年第21期2240-2243,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30672601)

摘　　要：目的：利用RP-HPLC对金银花和连翘单煎、单煎后合并及混煎进行分析，寻找异同。方法：采用Alltech-C18（4．6mm×250mm，5μm）色谱柱进行分离，流动相采用乙腈-水（含0．2％醋酸）系统梯度洗脱，流速1．0mL·min^-1，检测波长有280nm，柱温为室温，进样体积10μL。结果：绿原酸和连翘酯苷A分别在下列范围内有良好的线性：0．980—9．800μg（r=0．9999）和0．972—9．720μg（r=0．9999）。平均回收率（n=6）分别为99．85％（RSD2．0％）和100．87％（RSD1．6％）。归属了银翘药对主要色谱峰的来源，定量了绿原酸和连翘酯苷A的含量，定性地比较了以下色谱峰所代表的成分在4个供试品中含量的多少，色谱峰k，m，n：金银花〉合并〉混煎；色谱峰g，h：连翘〉合并〉混煎；色谱峰d，e，i：混煎〉金银花，混煎〉合并。结论：金银花和连翘单煎、单煎后合并及混煎4个供试品的RP—HPLC图有异同表明银翘药对的物质基础和金银花、连翘单用的物质基础有异同，为进一步研究课题“银翘药对的药效物质基础研究”提供了方向，为临床和制药提供了一些实验依据，同时也为中药复方研究提供了参考，具有一定的理论和实践意义。Objective： Utilizing RP-HPLC analysis of single, merging and simultaneously boiled Lonicerajaponica and Forsythia suspense to find out their identity and difference. Method： Samples were separated by Alltech-C18 column （4. 6 mm ×250 mm, 5 μm） with a mixture solution of acetonitrile-water （containing 0. 2% ethanoic acid） as mobile phase, 1.0 mL· min^-1 as flow-rate, 280 nm as detected wave-length, room temperature as temperature of column, 10 μL as injected volume. Result： The linear ranges of chlorogenic acid and forsythoside A were 0. 980-9. 800 μg （ r = 0.999 9） and 0. 972-9. 720 μg （ r = 0.999 9）, respectively. The average recoveries of chlorogenic acid and forsythoside A were 99. 85% （n =6, RSD 2.0% ）and 100. 87% （n =6, RSD 1.6% ）, respectively. Major peaks were confirmed to the two component herbs, contents of chlorogenic acid and forsythoside A were determinated, ingredients were represented by following peaks were compared qualitatively： peaks k, m, n： L. japonica 〉 merging sample 〉 boiled sam- ple; peaks g, h： Forsythia suspense 〉merging sample 〉boiled sample; peaks d, e, i： boiled sample 〉L. japonica, boiled sample 〉 merging sample. Conclusion： Difference and identities among 4 experimental samples of RP-HPLC separations show that Yinqiao, L. japonica and F. suspense＂ substances are difference and identities, which offered direction for investigating further the topic-pharmacodynamic substance of Yinqiao, provided increasing experimental evidence for clinic medicine and pharmaceutics and supplied reference for the complex prescription of traditional Chinese medicine.

关 键 词：RP—HPLC 银翘药对 金银花 连翘 合并 混煎 中药复方 

分 类 号：R284.1[医药卫生—中药学]