金草片对子宫肌瘤模型大鼠免疫功能的干预作用  

Improvement effects of Jincao tablet on immune function of model of hysteromyoma in rats

作　　者：李建荣[1] 吴兰生[1] 张广平[1] 

机构地区：[1]中国中医科学院中药研究所

出　　处：《中国中药杂志》2007年第21期2277-2281,共5页China Journal of Chinese Materia Medica

基　　金：科技部创新药物和中药现代化项目(2002AA2Z3225)

摘　　要：目的:探讨金草片对子宫肌瘤模型大鼠免疫功能的干预作用及该模型与临床发病机制的相关性。方法:大鼠随机分为正常组、模型组、阳性药组,金草片高、中、低剂量组,以雌激素和雌、孕激素肌肉注射造成大鼠子宫肌瘤模型,观察模型大鼠胸腺、脾脏、子宫和卵巢质量,用流式细胞仪测定其T细胞亚群CD3,CD4和CD8的水平,用放射配体结合分析法测定雌激素受体水平,进行子宫组织的病理检查。结果:模型组大鼠表现出其免疫器官胸腺质量降低,子宫质量增加,卵巢质量降低;CD3,CD4和CD4/CD8明显降低,CD8明显升高;雌激素及其受体水平升高;子宫组织呈明显病变,金草片剂量组与模型组比较,检测各指标差异显著。结论:金草片对模型大鼠的免疫功能低下状态表现为明显对抗作用,调节免疫功能可能是其治疗子宫肌瘤的作用机制之一。Objective： To investigate the improvement effects of Jincao tablet on immune function of the model of hysteromyoma in rat and the relationship between the model and pathogenesis. Method： Rats were randomly divided into 6 groups： normal group, model group, treatment groups including low, middle and high dosage groups of Jincao tablet and Guizhi Fuling pill. Rats were injected respectively with diethyl stilbestrol and progesterone. The immune apparatus of rats were measured. The levels of CD3, CD4 and CD4/ CD8 in serum were determined by flow cytometer. The estrogen and receptor were measured by radioligand binding assay and pathologic changes of womb tissue were observed microscopically. Result： Compared with normal group, the weight of thymus, the levels of CD3, CD4 and CD4/CD8 of model group were significantly decreased, and the levels of estrogen, estrogen receptor and CD8 were obviously increased. Jincao tablet groups were significant difference compared with model group and could alleviate the pathological changes of womb tissue. Conclusion： Jincao tablet could improve the levels of immune function of the model of hysteromyoma in rat, and it might play a role in the pathogenesis of leiomyoma.

关 键 词：金草片 大鼠 子宫肌瘤模型 T细胞亚群 雌激素受体 

分 类 号：R285.5[医药卫生—中药学]