自发性高血压大鼠心肌TGF-β-1的表达及厄贝沙坦和咪哒普利的影响  

Effects of Imidapril and Irbesartan on the expression of transforming growth factor-β_1 in the left ventricular myocardium of spontaneously hypertensive rats

作　　者：朱中生[1] 王晋明[2] 

机构地区：[1]南京医科大学附属南京第一医院心内科,江苏南京210006 [2]武汉大学人民医院心内科

出　　处：《中国医科大学学报》2007年第5期514-516,共3页Journal of China Medical University

基　　金：湖北省教育厅自然科学基金资助项目(301140080)

摘　　要：目的:探讨转化生长因子β(1TGF-β1)在自发性高血压大鼠(SHR)左心室肥厚发生过程中的作用和咪哒普利、厄贝沙坦在抑制TGF-β1表达方面的作用效果。方法:选用13周龄SHR30只,随机分为SHR组、厄贝沙坦组、咪哒普利组;另选同源同系WKY大鼠10只,作为正常对照组(WKY组)。应用大鼠尾压心率测定仪测量血压,应用SABC免疫组化及RT-PCR检测左心室肌TGF-β1蛋白及mRNA水平。结果:血压、左心室肌TGF-β1蛋白和mRNA的表达SHR组明显增加;咪哒普利组、厄贝沙坦组降低;咪哒普利组的表达低于厄贝沙坦组。结论:SHR的左心室肌TGF-β1蛋白和mRNA的表达明显增高;咪哒普利、厄贝沙坦不仅能良好地控制血压而且可以抑制SHR左心室肌TGF-β1蛋白和mRNA的表达;咪哒普利对TGF-β1蛋白和mRNA表达的抑制作用强于厄贝沙坦。Objective：To explore the role of transforming growth factor-β1 （TGF-β1） in the development of left ventricular hypertrophy in spontaneously hypertensive rat （SHR） and the effects of imidapril and irbesartan on TGF-β1 expression. Methods：Thirty 13-week-old SHRs were randomly divided into SHR group,imidapril group,and irbesartan group,and 10 homogenous Wistar-Kyoto （WKY） rats were selected as normal control group. The blood pressure of rats was monitored. The levels of TGF-β1 protein and mRNA were determined by immunohistochemical SABC method and reverse transcription polymerase chain reaction, respectively. Results：The blood pressure and the levels of TGF-β1 protein and mRNA significantly increased in SHR group and decreased in imidapril and irbesartan groups. The levels of TGF-β1 protein and mRNA in imidapril group were lower than those in irbesartan group. Conclusion：imidapril and irbesartan could not only control blood pressure but also inhibit the expressions of TGF-β1 protein and mRNA in the left ventricle myocardium of SHR. Such effects are stronger in imidapril group than in irbesartan group.

关 键 词：自发性高血压大鼠 咪哒普利 厄贝沙坦 转化生长因子Β1 

分 类 号：R544.1[医药卫生—心血管疾病]