激素对急性肺损伤大鼠BALF及血清SP-A的影响  

The preliminary study on the effect of steroid on SP-A in BALF and serum of ALI rats

作　　者：焦光宇[1] 张晓晔[1] 刘春利[1] 高红[2] 何平[3] 

机构地区：[1]中国医科大学附属盛京医院呼吸内科,辽宁沈阳110004 [2]中国医科大学卫生部小儿先天畸形实验室 [3]中国医科大学附属盛京医院干诊科,辽宁沈阳110004

出　　处：《中国医科大学学报》2007年第5期520-522,共3页Journal of China Medical University

基　　金：辽宁省博士启动基金资助项目(20021033);沈阳市青年启动基金资助项目(2003-48)

摘　　要：目的:通过对急性肺损伤大鼠支气管肺泡灌洗液(BALF)及血清标本表面活性蛋白A(SP-A)的检测,并加用糖皮质激素干预观察对SP-A的影响,研究SP-A在急性肺损伤发病过程中的作用。方法:肺损伤实验组采用脂多糖气管内灌注大鼠制备急性肺损伤动物模型,干预组气管内灌注脂多糖的同时尾静脉推注甲基强的松龙;分别采集4,12,24h组BALF及血清样本,采用斑点杂交方法分析SP-A的含量。结果:BALF中SP-A的含量于肺损伤4～24h组均明显低于对照组;激素干预4～12h组SP-A含量明显高于肺损伤组。血清SP-A于损伤后4～24h均明显增高;激素干预4～12h组较肺损伤组血清SP-A明显下降。相关分析表明血清与BALF中SP-A的含量呈明显的负相关关系。结论:BALF中SP-A的含量降低可以早期准确地反映肺泡毛细血管膜的损伤程度;血清SP-A含量可以作为早期敏感的周围血标志物,反映肺损伤的情况。在急性肺损伤的早期给予激素可能会减轻SP-A的破坏。Objective： To elucidate the role of suffactant protein A （SP-A） in the acute lung injury （ALI） by detecting the level of SP-A in bronchoalveolar lavage fluid （BALF） and serum and by observing the effect of steroid on SP-A in ALI rats. Methods： The ALI rat model for ALI group was established by instilling LPS into the trachea. Mythylprednisolone was also injected through the tail vein in the steroid treated group. The BALF and serum samples were collected at 4 h, 12 h and 24 h respectively and the content of SP-A was measured with immunoblot analysis. Results： The content of SP-A in BALF was decreased significantly in all ALI groups as compared with the control group （P 〈 0.05）; The content of SP-A in steroid treated groups was increased significantly as compared with the ALI groups （P 〈 0.05）; The content of SP-A in serum was increased in all ALI groups; The content of SP-A in sorum in 4-12 h of steroid treated groups was decreased significantly as compared with ALl groups （P 〈 0.05 ）. The correlation analysis showed the negative correlation of the content of SP- A in serum and in BALK Conclusion： The decrease of SP-A in BALF could indicate accurately the injury of alveoli capillaiy membrane in the early stage. The content of SP-A in serum could be a sensitive prognostic marker to reflect the injury of the lung. The administration of steroid may relieve the damage to SP-A in ALI.

关 键 词：急性肺损伤 表面活性蛋白A 糖皮质激素 

分 类 号：R563.8[医药卫生—呼吸系统]