bFGF对骨肉瘤细胞系U2-os细胞的促凋亡作用  

Effect of bFGF on the apoptosis of U2-os osteosarcoma cell line

作　　者：彭博[1] 吴哲[2] 张朝东[2] 

机构地区：[1]中国医科大学门诊部,辽宁沈阳110001 [2]中国医科大学附属第一医院神经内科

出　　处：《中国医科大学学报》2007年第5期526-528,共3页Journal of China Medical University

基　　金：辽宁省博士启动基金资助项目(20051035)

摘　　要：目的:观察碱性成纤维细胞生长因子(bFGF)对骨肉瘤细胞系U2-os细胞增殖及生存的影响,探讨bFGF在肿瘤细胞发生发展中的作用。方法:以bFGF处理U2-os细胞,采用台盼蓝排除检测法及MTT法检测细胞存活及增殖情况;用Annexin V-EGFP/PI双染流式细胞术及DNA梯状带检测细胞凋亡情况。结果:bFGF呈浓度、时间依赖性降低U2-os细胞活力并诱导细胞凋亡。结论:bFGF可以诱导U2-os细胞发生凋亡,生长因子在细胞的生长调控方面具有双重作用。Objective： To study the role of basic fibroblast growth factor （ bFGF ） in the generation and development of tumor by investigating the inhibition effect of bFGF on the proliferation and survival of U2-os osteosarcoma cell line. Methods：U2-os cells were treated by bFGF, and their survival and proliferation were detemtined with trypan blue exclusion method and MTT method, The apoptosis in U2-os induced by bFGF was determined by Annexin V-EGFP/PI staining of fixed cells and with FACS can flow cytometry and DNA ladder analysis. Results： bFGF decreased cell activity and proliferation and induced apoptosis in U2-os cells in dependency of concentration and time. Conclusion：bFGF can induce the apoptosis of U2-os osteosareoma cell line, and the growth factors have double regulative effects on cell growth.

关 键 词：骨肉瘤细胞系U2-os细胞 碱性成纤维细胞生长因子 凋亡 

分 类 号：Q255[生物学—细胞生物学]