caspase-3在脑室内低温动物模型脑组织中的表达  

Expression of caspase-3 in the brain tissue of rabbit model of ventricular hypothermia

作　　者：隋宇玲[1] 傅志坚[1] 王运杰[1] 姚长义[1] 贾志军 

机构地区：[1]中国医科大学附属第一医院神经外科,辽宁沈阳110001 [2]内蒙古赤峰市宁城县医院急诊科

出　　处：《中国医科大学学报》2007年第5期548-550,共3页Journal of China Medical University

摘　　要：目的:对家兔急性脑出血模型进行脑室内低温保护,通过对动物行为和对出血灶周围脑组织天冬氨酸特异性半胱氨酸蛋白酶3(caspase-3)表达的观察,探讨脑室内低温对家兔急性脑出血模型细胞凋亡的影响。方法:4～6月龄雄性家兔12只,随机分为等温组、低温组各6只。于右侧内囊点注入耳中动脉血0.5ml制作脑出血模型。用双腔微导管穿刺左侧侧脑室,建立脑室内灌注装置,以等温/低温等渗盐水灌注2h。术后24h观察其肢体运动后取材、固定、染色,观察脑组织caspase-3表达情况。结果:经脑室内低温保护的动物肢体偏瘫程度轻,血肿周围脑组织内出现的caspase-3阳性细胞数量较少,与对照组的差异具有统计学意义(P<0.05)。结论:对家兔急性脑出血模型进行脑室内低温脑保护可以减少血肿周围脑组织的细胞凋亡,脑室内低温作为选择行脑部低温的一个新的思路值得关注。Objective：To investigate the effect of ventricular hypothermia on the cell apoptosis in rabbit model of acute intracerebral hemorrhage （ICH） by observing the animals＇ behavior and the expression of caspase-3 in the brain tissues immediately adjacent to the hematoma. Methods：Twelve male rabbits of 4 to 6 months old were randomly and equally divided into normothermia group and hypothermia group. The ICH model was established by injecting 0.5 ml of self-arterial blood obtained from middle ear artery into the right internal capsule. A double-cavity cannula was punctured into the left lateral ventricle, and then the normothemic or hypothemic normal saline was perfused into the lateral ventricle for 2 hours. The aniraals＇ behavior was observed after 24 hours, and the brain tissues were sampled, fixed in 4% paraformaldehyde, and stained. The expression of caspase-3 was detected. Results：Corapared with normothermia group, the number of caspase-3-positive cells was smaller and the neurological deficits were less sever in hypothermia group （P 〈 0.05 ）. Conclusion：Ventricular hypothermia can reduce the brain injury associated with ICH, which provides a promising therapeutic method for treating acute ICH.

关 键 词：继发性神经元损伤 细胞凋亡 天冬氨酸特异性半胱氨酸蛋白酶3 脑室内低温 

分 类 号：R651.1[医药卫生—外科学]