蛋白激酶B对小鼠1-细胞期受精卵卵裂的影响  

Effect of Protein Kinase B on Cleavage of Mouse Fertilized Eggs

作　　者：冯晨[1] 王雁[2] 宿文辉[1] 张哲[1] 徐小燕[1] 宗志红[1] 于秉治[1] 

机构地区：[1]中国医科大学基础医学院生物化学与分子生物学教研室,沈阳110001 [2]中国医科大学实验技术中心三部,沈阳110001

出　　处：《生殖与避孕》2007年第10期633-638,共6页Reproduction and Contraception

基　　金：国家自然科学基金项目(30570945)资助

摘　　要：目的:研究蛋白激酶B(PKB)的活性变化对小鼠受精卵早期发育的影响。方法:将野生型、持续激活型和激酶失活型PKB克隆至pBluescriptII/SK表达载体中,体外转录成mRNA,显微注射到1-细胞期受精卵中,观察受精卵分裂情况。结果:1-细胞期受精卵注射野生型或持续激活型PKB的mRNA后,卵裂率均增加,而持续激活型PKB的促进作用更为显著;相反注射激酶失活型PKB的mRNA后,卵裂率明显降低。结论:PKB能促进小鼠1-细胞期受精卵的发育,其活化对于小鼠受精卵的发育是必需的。Objective： To investigate the effect of protein kinase B（PKB） on the regulation of mouse early embryo development. Methods： Wild type, constitutively active and kinase dead PKB（PKB-KD） were cloned and inserted into the vector pBluescript II/SK, transcribed into mRNA in vitro and then microinjected into mouse 1-cell stage fertilized eggs to observe the mitotic division of eggs. Results： Injection of mRNA coding for a constitutively active PKB（myr-PKB） into 1-cell stage fertilized eggs induced cell division more effectively than injection of wild type PKB（PKB-WT） mRNA, whereas microinjection ofmRNA of PKB-KD delayed the first mitotic division. Conclusion： PKB can promote the division of mouse 1-cell stage fertilized eggs. The activation of this kinase is necessary for the first round mitosis of mouse embryos.

关 键 词：小鼠受精卵 蛋白激酶B(PKB) 定点突变 

分 类 号：Q954.4[生物学—动物学]