生长激素对小鼠子宫内膜VEGF、LIF、MMP-9及TIMP-1表达的影响  

Effect of GH on the Expressions of VEGF,LIF,MMP-9 and TIMP-1 in Endometrium of Mouse

作　　者：项云改[1] 谭丽[1] 董方莉[1] 

机构地区：[1]郑州大学第二附属医院生殖中心,郑州450014

出　　处：《生殖与避孕》2007年第10期639-643,共5页Reproduction and Contraception

摘　　要：目的:探讨生长激素(GH)对子宫内膜容受性的作用。方法:将150只小鼠随机平均分成3大组:A组(动情后期组)、B组(假孕组)、C组(妊娠d4组);每大组再分为2亚组:第1亚组皮下注射GH1.5mIU/g,第2亚组注射等体积生理盐水;用免疫组化技术检测子宫内膜VEGF、LIF、MMP-9及TIMP-1的表达。结果:B组子宫内膜中,注射GH者LIF、VEGF、MMP-9及TIMP-1的表达呈阳性,强于其对照组,二者比较差异有显著性(P均<0.05);C组子宫内膜中,注射GH者LIF、VEGF、MMP-9及TIMP-1的表达呈强阳性,强于其对照组,二者比较差异有显著性(P均<0.05)。结论:GH可以提高小鼠着床期子宫内膜VEGF、LIF、MMP-9及TIMP-1的表达,从而改善子宫内膜容受性,提高临床妊娠率。Objective： To study the effect of GH on endometrial receptivity. Methods： A total of 150 mice were divided into 3 groups randomly： metestrus stage （group A）, pseudopregnant mice （group B）, the 4th day ofpregnant mice （group C）. Each group was divided into 2 subgroups： one was given 1.5 mIU/g GH （group 1） and the other received physiological saline in equal volum （group 2）. The expressions ofVEGF, LIF, MMP-9 and TIMP-1 of the 6 groups were examined by immunohistochemistry techniques in mouse endometrium. Results： The expressions of VEGF, LIF, MMP-9 and TIMP-1 in the group B2 were lower than those in the group B1, the difference between them were significant （P〈0.05）; more stronger expressions of VEGF, LIF, MMP-9 and TIMP-1 were found in the group C1 than in the group C2, the difference between them were significant （P〈0.05）. Conclusion： GH can inhance the expressions of VEGF, LIF, MMP-9 and TIMP-1 in endometrium of mouse during implantation window phase. Using GH may improve the endometrial receptivity and further the rate of pregnancy.

关 键 词：生长激素(GH) 内膜容受性 白血病抑制因子(LIF) 血管内皮生长因子( VEGF) 基质金属蛋白酶-9(MMP-9) 

分 类 号：R714[医药卫生—妇产科学]