液相芯片技术在人乳头瘤病毒检测和分型中的应用  

The application of liquid chips technology in human papilloma virus detection and genotyping

作　　者：刘思瑶[1] 丁显平[1] 朱一剑[1] 魏萍[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室遗传医学研究所,成都610064

出　　处：《四川大学学报（自然科学版）》2007年第5期1111-1114,共4页Journal of Sichuan University(Natural Science Edition)

摘　　要：从50例疑似感染人乳头瘤病毒(Human Papillomavirus,HPV)患者的标本中提取DNA,运用液相芯片技术对提取的DNA进行7种低危型和19种高危型检测,并与反向点杂交检测结果进行比较.50例标本中,液相芯片检出阳性26例,阳性率为52%,其中单一亚型感染20例占40%,多重感染6例占12%,比反向点杂交的多重感染阳性检出率高4%.50 specimens from those suspected for HPV infection were detected with the method of liquid chips and reverse line blot assay. In all 50 specimens, 26（52%） were detected positive for HPV DNA with the method of liquid chips, including 20（40% ） single infection and 6（12% ） multiple infection. However, there were just 4（8 % ） multiple infection with the method of reverse line blot assay.

关 键 词：液相芯片 HPV 基因分型 

分 类 号：Q81[生物学—生物工程]