热性惊厥患儿血浆和外周血单个核细胞表面ICAM-1/LFA-1的表达水平  

Expression levels of ICAM-1 and LFA-1 in plasma and PBMC of children with febrile seizures

作　　者：刘智胜[1] 姚辉[2] 孙丹[1] 康世秀[2] 何彩英[1] 胡家胜[1] 吴革菲[1] 王芳琳[1] 

机构地区：[1]武汉市儿童医院神经内科,湖北武汉430016 [2]武汉市儿童医院中心实验室,湖北武汉430016

出　　处：《中国实用儿科杂志》2007年第11期826-829,共4页Chinese Journal of Practical Pediatrics

基　　金：武汉市青年科技晨光计划资助项目(项目编号20015005050)

摘　　要：目的探讨细胞间黏附因子-1(ICAM-1)及其配体淋巴细胞功能相关抗原-1(LFA-1)对热性惊厥(FS)患儿的神经免疫调节作用。方法2004-11—2006-12将武汉市儿童医院60例FS患儿分为单纯性FS(SFS)组30例和复杂性FS(CFS)组30例;对照组30例,为年龄和性别相匹配的同期体检健康儿童。采用双抗夹心ELISA法检测血浆可溶性ICAM-1/LFA-1水平,流式细胞术检测外周血单个核细胞(PBMC)表面ICAM-1/LFA-1的表达水平。结果SFS组和CFS组患儿血浆ICAM-1水平分别为(21.54±11.09)ng/mL和(24.34±6.86)ng/mL,均明显低于对照组(29.73±12.39)ng/mL儿童(P<0.05);3组血浆LFA-1水平从高到低依次为CFS组(12.30±8.04)ng/mL、SFS组(12.09±8.83)ng/mL和对照组(9.51±8.07)ng/mL,组间比较差异无统计学意义(P>0.05)。SFS组的PBMC表面ICAM-1表达水平为(29.96±12.31)%,明显高于CFS组(22.50±8.19)%及对照组(14.21±11.31)%儿童(P<0.05),CFS组的PBMC表面ICAM-1表达水平明显高于对照组儿童(P<0.05);而LFA-1在PBMC表面的表达水平则不同,3组比较,SFS组最高为(50.89±21.36)%,CFS组最低为(34.35±11.45)%,对照组为(41.39±16.30)%,组间比较差异有统计学意义。结论ICAM-1/LFA-1作为早期应激状态下的协同刺激信号免疫因子,参与白细胞黏附及其黏附级联反应,使大脑神经元在对热应激不适应基础上呈过度兴奋状态,诱导惊厥的发生。且CFS的神经免疫病理过程要比SFS相对复杂,抑制FS的ICAM-1/LFA-1黏附活动可能为其防治开辟新的途径。Objective To explore the neuroimmunomodulation mechanism of ICAM-1 and LFA-1 in children with febrile seizures （FS）. Methods Sixty children with FS were divided into simple FS （SFS） group（30） and complex FS （EFS） group （ 30 ） , and control group of 30 health children was matched with regard to age and sex. Adopting enzyme-linked immunosorbentassay （ELISA） and flow cytometry （ FCM ） ,we measured the levels of ICAM-1 and LFA-1 contained in plasma and peripheral blood mononuclear cell （ PBMC ） of children with FS and control.group. Results The plasma levels of soluble ICAM-1 （ sICAM-1 ） in SFS group [ （21.54 ± 11.09） ng/ml] and CFS group [ （24. 34 ±6.86） ng/ml] were significantly lower than that in the control group [ （ 29.73 ± 12. 39 ） ng/ml ] （ P 〈 0. 05 ）. The plasma soluble LFA-1 （ sLFA-1） levels of CFS,SFS and control groups were （12.30 ±8.04） ,（ 12.09 ±8.83） and（9. 51 ± 8.07） ng/ml respectively, but there was no statistical difference between various groups （ P 〉 0. 05 ）. The PBMC levels of ICAM-1 were significantly higher in SFS group [ （29. 96 ± 12. 31 ） % ] than those in CFS group [ （22. 50 ± 8. 19） % ] and control group [ （ 14. 21 ± 11.31 ） % ] （ P 〈 0. 05 ） ; at the same time, there was significantly higher expression in CFS group than in control group （ P 〈 0. 05）. However,the expression levels of LFA-1 was different from the ICAM-1 in the surface of PBMC. Of the three groups, the highest LFA-1 level was in the SFS group [ （ 50. 89 ± 21.36 ） % ], the lowest LFA-1 level was in the CFS group [ （34. 35 ± 11.45 ）% ] and [（41.39 ± 16. 30）%] was the LFA-1 level of control group in PBMC. Significant difference was found in three groups （ P 〈 0. 05 ）. Conclusion As a kind of costimulatory immunologic molecule under early stress, ICAM-1/LFA-1 participate in leukocytic adherence and cascade reaction of adherence ,which results in overexcitation of neurons on base of unadapting to fever

关 键 词：热性惊厥 黏附因子 ICAM-1 LFA-1 

分 类 号：R72[医药卫生—儿科]