hIGF-1基因在退变椎间盘中的表达  

Human insulin-like growth factor-1 gene expression on intervertebral disk degeneration

作　　者：黄宗强[1] 刘尚礼[2] 郑召民[3] 

机构地区：[1]郑州大学第一附属医院骨科,郑州450052 [2]中山大学附属第二医院骨科,广州510120 [3]中山大学附属第一医院骨科,广州510089

出　　处：《中国矫形外科杂志》2007年第21期1651-1653,共3页Orthopedic Journal of China

基　　金：国家自然科学基金资助项目(NO.30471746);广东省科委攻关项目(NO.C30702)

摘　　要：[目的]探讨hIGF-1基因在退变椎间盘中的表达。[方法]参考文献制备出椎间盘退变（intervertebral disk degeneration，IVDD）动物模型24只，随机分为Ad／CMV—hIGF-1、hIGF-1生长因子及PBS组，每组有新西兰大白兔8只。L4,5、L5,6椎间盘中分别注射第2代Ad／CMV—hIGF-1（8×10^8PFU）、hIGF-1生长因子（100μg／L）、PBS25μL。注射后1、2、4、8周，Western blot检测椎间盘中hIGF-1蛋白表达。[结果]hIGF-1蛋白带出现在7539．96μ。Ad／CMV—hIGF-1、hIGF-1蛋白表达持续达4周以上，hIGF-1组表达持续约2周；PBS注射组无hIGF-1蛋白表达。[结论]hIGF-1基因能够在退变椎间盘中表达；hIGF-1基因转染表达的持续时间长于单纯hIGF-1生长因子直接注射。[Objective] To study hIGF-1 gene expression on intervertebral disk degeneration. [ Method] Twenty-four male New-Zealand rabbits IVDD models were made according to reference and randomly divided into Ad/CMV-hIGF-1, hIGF-1 growth factor and PBS group. Twenty five microlitre the second generation Ad/CMV-hIGF-1 （T = 80 × 10^9 PFU/L）, hIGF-1 growth factor （100 μg/L） and PBS were respectively injected into L4.5, L5,6 intervertebral disk under fluoroscopic guidance. One, two, four and eight weeks post-operation, rabbits were sacrificed, intervertebral disk samples were harvested. Total proteins of equal mass intervertebral disks were extracted, isolated in SDS-polyacrylamide gel electrophoresis （SDS-PAGE） and transferred to polyvinylidene difluoride （PVDF） Millipore. The hIGF-1 growth factor expression were indentified with Western blot. [ Result] The hIGF-1 interest protein existed at 7.6 kilo-Dalton. At one week after injection, its expression quantities were almost equal between Ad/CMV-hIGF-1 and hIGF-1 growth factor group. At two week after injection, it obviously declined in hIGF-1 growth factor group. At four week after injection, it still expressed in Ad/CMV-hIGF-1 group. At eight week after injection, it did not express in three groups. [ Conclusion ] Ad/CMV-hIGF-1 successfully infects degenerative intervertebral disk. In Ad/CMV-hIGF-1 group, the hIGF-1 gene expression lasts longer than that in hIGF-1 growth factor group.

关 键 词：椎间盘 退变 HIGF-1 

分 类 号：R681.5[医药卫生—骨科学]