中国李Nubiana离体叶片愈伤组织诱导的研究  

Callus Inducement in vitro Culture of Chinese Plum Nubiana Leaves

作　　者：樊青峰[1] 李国怀[1] 浦艳吉[1] 姚延兴[1] 

机构地区：[1]华中农业大学园艺林学学院,武汉430070

出　　处：《华中农业大学学报》2007年第5期680-683,共4页Journal of Huazhong Agricultural University

基　　金：湖北省重点科技攻关项目(2001AA209B-140)资助

摘　　要：以Nubiana叶片为试材,对其愈伤组织的诱导、继代培养及褐变调控进行了研究。结果表明：Nubiana叶片愈伤的诱导及继代培养以WPM附加BA 0.5 mg/L和2.4-D 2.0 mg/L的培养基上效果最好;0.1%的柠檬酸（CA）具有明显地促进愈伤组织生长、抑制褐变的作用,而聚乙烯吡咯烷酮（PVP）的效果最差;3%的葡萄糖（Glu）最适于愈伤组织的诱导及继代。综合以上研究结果,获得较好的愈伤组织的适宜培养条件是：WPM＋BA 0.5 mg/L＋2,4-D 2.0 mg/L＋Glu 3%＋CA 0.075%-0.1%,暗培养28-30 d。The inducing, subculture, and control browning of callus in leaves of Nubiana were studied in order to establish the optimum condition of inducing and growth of callus. The results showed that WPM medium supplemented with BA 0. S mg/L and 2,4-D 2.0 mg/L was the best medium for the inducing and subculture of callus; 0. 1% of citric acid（CA） had remarkable results of promoting the growth and controlling the browning of callus, while the PVP showed the worst result; 3%-5% of glucose was the best carbon source of inducing and subculture of callus. It can be concluded that the suitable culture condition to produce a high mass was WPM medium supplemented with BA 0. 5 mg/L, 2,4-D 2.0 mg/L,glucose 3% and CA 0. 075%-0. 100% for 28-30 days in darkness.

关 键 词：Nubiana 愈伤组织诱导 葡萄糖 柠檬酸 

分 类 号：S662.303.6[农业科学—果树学]