百日草自交系花粉萌发条件及花粉活力研究  

In vitro Pollen Germination of the Inbred Lines of Zinnia elegans Jacq.

作　　者：叶要妹[1] 张佳祺[1] 张双凤[1] 包满珠[1] 

机构地区：[1]华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室,武汉430070

出　　处：《华中农业大学学报》2007年第5期693-696,共4页Journal of Huazhong Agricultural University

基　　金：国家"948"项目(2003-Z36)资助

摘　　要：研究了不同培养基、采集时间、贮藏温度和湿度等因素对百日草花粉离体培养萌发效果的影响。结果表明:花粉萌发较适宜的培养基为ME3+16%PEG4000+12%蔗糖;上午10:00采集花粉萌发率要显著地高于下午3:00采集的;在4℃湿润条件下贮藏18 h,J6花粉生活力下降较小,而在室温干燥条件下几乎失去活力;相对湿度在66%以下时极不利于花粉保存;11个百日草自交系花粉离体萌发率差异显著。The factors on different culture medium, collecting time, storage temperature and relative humidity affecting pollen germination of Zinnia elegans were studied. The results are as follows：The optimum germination medium in vitro pollen germination of Zinnia elegans is a basic medium ME3 supplemented with 16% PEG4000 and 12% sucrose. The pollens germination rate which pollens is collected at 10：00 am is signifieantly different to that at 3.00 pm. The pollen germination rate of J6 reduced more little at 4 ℃ and moist for 18 h,but it almost lost all at room temperature and dry. Pollen stored very bad below 66% of relative humidity. Pollens germination of 11 Zinnia elegans cultivars cultured on the medium are significantly different.

关 键 词：百日草 自交系 离体花粉 萌发 花粉生活力 

分 类 号：S688.4[农业科学—观赏园艺]