一个家蝇抗菌肽的分离纯化  

Purification of an Antibacterial Peptide from Musca domestica Larvae

作　　者：钟雅[1] 付康[1] 陈正望[1] 陆婕[1] 

机构地区：[1]华中科技大学生命科学与技术学院,武汉430074

出　　处：《华中农业大学学报》2007年第5期727-729,共3页Journal of Huazhong Agricultural University

基　　金：国家"863"计划项目(2002AA214061);国家自然科学基金项目(30370647)资助

摘　　要：以野生家蝇干燥幼虫为原料,在常规的生化提取基础上,通过反相高效液相色谱(RP-HPLC)法进一步纯化,并结合电洗脱技术,利用灵敏的杀菌活性检测手段,从家蝇抗菌肽总组分中分离纯化出1个20 ku的抗菌肽,对苏云金芽孢杆菌、枯草芽孢杆菌、金黄葡萄球菌均有杀灭作用。Based on the routine biochemical separating techniques, using RP-HPLC and electro-elution, a 20 ku antibacterial peptide had been purified from Musca domestica dry larvae detected by a highly sensitive bactericidal method. The peptide has bioactivity against Gram-positive bacteria such as Bacillus thuringiensis,Bacillus subtilis and Staphylococcus aureus. The research has provided theoretic foundation for purifying antibacterial peptides using preparative scale RP-HPLC.

关 键 词：家蝇蛆 抗菌肽 抗菌活性 反相高效液相色谱 电洗脱 

分 类 号：Q516[生物学—生物化学]