应用氧化棉织物固定过氧化氢酶的研究  

Immobilization of catalase on oxidized cotton fabric

作　　者：厉成宣[1] 范雪荣[1] 王强[1] 王钦清[1] 

机构地区：[1]江南大学生态纺织教育部重点实验室,江苏无锡214122

出　　处：《印染》2007年第21期1-4,共4页China Dyeing and Finishing

基　　金：本课题得到国家高技术研究发展计划(863计划)资助(课题编号:2007AA02Z218)

摘　　要：以高碘酸钠氧化棉织物为载体固定过氧化氢酶,研究其固定化的主要条件,考察了固定化酶的性质。试验结果表明,过氧化氢酶固定在氧化棉织物上时,棉织物的最佳氧化条件为:NaIO_4浓度0.20 mol/L,氧化时间8h,氧化浴pH值6.0,氧化温度40℃;过氧化氢酶固定化的条件为:过氧化氢酶用量0.20 mL/(g棉织物),固定化时间24 h。固定化酶的最适温度比游离酶提高了约10℃,使用温度范围变宽;固定化酶的最适pH值为7.0与游离酶相同,在酸性范围内稳定性较游离酶差,但在弱碱性范围内稳定性比游离酶好。Immobilizing process of catalase on sodium periodate oxidized cotton fabric was carried out, optimum conditions were defined. Cotton fabric was oxidized by 0.20 mol/L sodium periodate at 40℃ for 8 hours under liquor pH value 6.0. Catalase with concentration of 0.20 mol/（g cotton fabric）was fixed on cotton fabric for 24 hours. Applied conditions of immobilized catalase were compared to those of free catalase. It was found that the former one had wider application temperature range, similar applied pH value, better stability under alkaline solution, while poorer stability in acidic solution.

关 键 词：染整 过氧化氢酶 氧化 高碘酸钠 棉织物 

分 类 号：TS190.641[轻工技术与工程—纺织化学与染整工程]