利用RNA干扰技术获得晚抽薹开花转基因大白菜(英文)  

Late-Bolting Transgenic Chinese Cabbage Obtained by RNA Interference Technique

作　　者：夏广清[1] 朱俊义[1] 何启伟[2] 赵双宜[3] 王翠花[2] 

机构地区：[1]通化师范学院生物系,吉林通化134001 [2]山东省农业科学院蔬菜研究所,济南250100 [3]山东大学生命科学学院,济南250100

出　　处：《植物生理与分子生物学学报》2007年第5期411-416,共6页Journal Of Plant Physiology and Molecular Biology

基　　金：山东省自然科学基金(No.Y2004D02)资助。~~

摘　　要：LEAFY基因在植物的成花转变中起重要作用。在拟南芥中,降低LEAFY基因的表达使开花延迟,并且增加分枝。利用RNAi技术降低大白菜LEAFY基因表达,获得晚抽薹开花的转基因大白菜。开花时转基因大白菜的株高明显低于对照,而叶片数、叶面积和侧枝数则高于对照;转基因植株的LEAFY基因表达明显降低,导致大白菜抽薹开花延迟。因此可以通过降低LEAFY基因表达来延迟需低温春化作用长日植物开花。也为利用生物技术获得晚抽薹转基因大白菜提供了理论依据。LEAFY （LFY） gene plays an important role in determining plant flowering mainly by controlling the timing of phase transition. Constitutive under-expression of LFY in Arabidopsis resulted in the formation of a late- flowering and highly branching phenotype. In this paper, an RNAi approach was used in down-regulated LFY gene expression to delay Chinese cabbage （Brassica rapa L. ssp. pekinensis） bolting and flowering. The results show that transgenic plant has a later transition to the repro- ductive phase, and the transgenic plants have more branches, more leaves, but a lower height. Results of RQ- RT-PCR analysis show that LFY gene expression was greatly reduced in transgenic plants. These results sug- gest that inhibiting LFY gene expression by RNA inter- ference can delay bolting in a cold-sensitive long-day （LD） condition. Late flowering of Chinese cabbage can be used as a good genetic resource for the breeding late bolting Chinese cabbage

关 键 词：大白菜 LEAFY基因 RNAI 抽薹开花 

分 类 号：S634.1[农业科学—蔬菜学]