NaCl胁迫对转基因杨树外源基因表达的影响  

Effects of NaCl Stress on the Exogenous Gene Expression in Transgenic Poplar Plants

作　　者：韩会景[1] 张法勇 杨敏生[1] 梁海永[1] 张华新[3] 

机构地区：[1]河北农业大学林学院,保定071000 [2]北京中林伟业科技有限公司,北京100714 [3]中国林业科学院林业研究所,北京100091

出　　处：《植物生理与分子生物学学报》2007年第5期435-440,共6页Journal Of Plant Physiology and Molecular Biology

基　　金：科技部"十一五"科技支撑计划(No.2006BAD03A0106);国家林业局科技推广项目(No.200603)资助。~~

摘　　要：以具高抗虫性的转抗虫基因‘741杨’及在此基础上转入了发根农杆菌Ri质粒T-DNA株系的组培苗为材料,研究了转基因株系BtCrylAc抗虫基因和发根基因的表达及其对NaCl胁迫的反应。结果表明,转入Ri质粒T- DNA上的rol基因后,导致苗木根系数目增加,根系长度减小,IAA和GA含量显著提高,抗虫BtCrylAc基因编码的毒蛋白的表达量降低;随着NaCl胁迫强度的增加,苗高、根系数量、叶绿素含量及IAA、GA含量逐步降低,而根系的长度加大,Bt毒蛋白含量显著提高,表明NaCl胁迫使转基因杨外源Bt毒蛋白基因的表达增强,而发根农杆菌Ri质粒T-DNA的表达下降。The expressions of BtCrylAc insect-re- sistance genes and rhizogenesis genes and their re- sponse to NaC1 stress were studied using tissue culture plants of high transgenic insect-resistant ‘poplar 741＇ and transpolygenes 741 （insect-resis- tant genes and T-DNA of Ri plasmid）. The results showed that IAA and GA contents increased quickly, plant root number increased and root length reduced after rhizogenesis and hormone synthesis related gene in Ri T-DNA were inserted into the genome of poplar （Figs.2, 3, 8 and 9）. Plant height, root number, chlorophyll content, IAA and GA contents decreased gradually with an increase in NaC1 stress intensity （Figs. 1, 2, 4-6, 8 and 9）. Apiece index change extent of transgenic rol geneplant was smaller than transgenic Bt gene plant and non-transgenic plant. Bt toxin protein content of transgenic rol gene plant increased significantly under NaC1 stress （Fig.7）. Our results indicate that the expressions of the foreign genes changed with the changes of the environmental conditions.

关 键 词：转基因植株 外源基因 NACI胁迫 毒蛋白 内源激素 

分 类 号：S792.11[农业科学—林木遗传育种] Q943[农业科学—林学]