水稻品种‘沈农606’抗稻瘟病基因定位和连锁的SRAP片段分析  

Blast Resistance Gene Mapping and Linked SRAP Fragment Analysis of Rice (Oryza sativa L.)‘Shennong606’

作　　者：姜树坤[1] 张喜娟[2] 张丽[1] 

机构地区：[1]沈阳农业大学辽宁省农业生物技术重点实验室 [2]辽宁省北方粳稻育种重点实验室,沈阳110161

出　　处：《植物生理学通讯》2007年第5期852-856,共5页Plant Physiology Communications

基　　金：辽宁省重点实验室专项资金计划;沈阳农业大学青年教师基金

摘　　要：选用抗稻瘟病水稻品种‘沈农606’为抗病亲本与感病品种‘丽江新团黑谷’配制杂交组合.鉴定亲本、F_1正反交及其F_2群体的抗病性的结果表明,‘沈农606’的抗性受一对显性基因控制.采用相关序列扩增多态性(SRAP)和简单序列重复(SSR)标记,以及分离体分组混合分析法(BSA)将该基因定位于8号染色体上,其与SRAP标记m5e1-500的遗传距离为2.8 cM,与SSR标记RM25的遗传距离为9.8 cM,暂命名为Pi-SN606.m5e1-500序列位于8号染色体上,它能编码大于40个氨基酸的阅读框有2个,在NCBI网站上没有比对到同源性序列。The rice （Oryza sativa） blast resistance cultivar ‘Shennong 606＇ was crossed to a susceptible cultivar ‘Lijiangxintuanheigu＇. Based on the segregation ratios of resistant and susceptible plant in F2 populations, it was deduced that the rice blast resistance gene was encoded by dominant gene. We report here the use of bulked segregate SRAP （sequence-related amplified polymorphism） and SSR （simple sequence repeat） analysis for rapid identification of DNA markers linked to the resistance gene. Two SRAP markers and one SSR marker were identified linking to the resistance gene locus. The linkage analysis with three markers using Mapmaker 3.0 software showed that the resistance gene locus was mapped to m5el-500 （2.8 cM） tightly and RM25 （9.8 cM）. Sequenced the m5el-500 fragment linked to the resistance gene and compared with the NCBI databases, the result proved the gene mapped on chromosome 8. Two ORFs （open reading frame） which was larger than 40 amino acids were coded by the m5el-500 sequence. This region does not align with the other plants in NCBI website.

关 键 词：水稻 稻瘟病 基因定位 SRAP SSR 

分 类 号：S511[农业科学—作物学]