蝴蝶兰花梗节间切段的类原球茎诱导与增殖  

Protocorm-like Body Induction and Propagation of Phalaenopsis by Using the Cutting Segment of Lower-stem-knot as Explants

作　　者：苏家乐[1] 陈尚平[1] 汤久顺[2] 何小弟[2] 李晓刚[1] 姜维华[2] 

机构地区：[1]江苏省农业科学院园艺研究所,南京210014 [2]扬州大学农学院,江苏扬州225009

出　　处：《植物生理学通讯》2007年第5期876-878,共3页Plant Physiology Communications

基　　金：江苏省农业三项工程项目[SX(2006)118]

摘　　要：采用不同基本培养基和生长调节物质浓度组合,诱导蝴蝶兰花梗节间切段形成类原球茎和增殖的结果表明,花梗可见后10 d的切段诱导效果较好,较适宜的培养基为N_6+5.0 mg·L^(-1)6-BA+0.2 mg·L^(-1)KT+2 mg·L^(-1)活性炭。活性炭控制外植体褐化的效果明显优于维生素C。类原球茎增殖的适宜培养基为1/3MS+0.3mg·L^(-1)TDZ+0.2mg·L^(-1)NAA+2g·L^(-1)活性炭。The cutting segment of lower-stem-knot of the Phalaenopsis was used as explant to study PLB （protocorm-like body） induction and propagation by different basic medium formula with growth regulation substance. The result showed that the induction effect was the best at 10 d among cutting section of flowers- stem-knot from different growth phase, N6 medium supplemented with 6-BA 5.0 mg·L^-1＋KT0.2mg·L^-1＋AC2g·L^-1 was the optimum medium for PLB induction, and AC could keep less browning than Vitamin C. 1/3MS medium supplemented with TDZ 0.3 mg·L^-1＋NAA 0.2mg·L^-1＋AC2g·L^-1 was the optimum medium for propa- gation of PLB.

关 键 词：蝴蝶兰 类原球茎 诱导 增殖 

分 类 号：S682.31[农业科学—观赏园艺]