温度胁迫对豇豆种子萌发生理指标的影响  

The Effect of Temperature Stress on Seed Germination Physiological Indices in Asparagus Bean(Vigna unguiculata L.ssp.sesquipdalis Verdc.)

作　　者：万茜[1] 刘伟[1] 陈禅友[1] 

机构地区：[1]江汉大学生命科学学院,湖北武汉430056

出　　处：《种子》2007年第10期32-35,共4页Seed

基　　金：武汉市科技攻关项目(20062002043)

摘　　要：以3个豇豆品种为材料,采用完全随机设计,重复3次,设置低温15℃、常温25℃(对照)和高温35℃3个处理,进行种子萌发实验。确定供试种子批种子活力正常后,在萌发种子胚根长到1 cm时,分胚根和子叶两个部位取样测定其可溶性蛋白质含量(TSP)、丙二醛(MDA)含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性等生理指标。结果表明:温度胁迫使胚根中TSP含量均较常温显著或极显著降低,子叶中的TSP含量在低温处理下极显著高于对照,而在高温下与之相反;温度胁迫下,各豇豆品种胚根和子叶中MDA含量均较显著或极显著高于对照;SOD活性变化与之相反,但品种间有差异。胚根POD活性在低温下极显著高于对照,在高温下却显著或极显著低于对照,而子叶中与之相反。子叶中CAT活性在低温下上升,高温下下降,而胚根中均上升。因此,低温和高温都明显阻碍豇豆种子萌发,但生理基础上不完全一致,且胚根和子叶的反应有明显差别,其中比较一致的是种胚萌发时MDA含量升高和SOD活性下降,反应不一致的有TSP含量、POD活性及CAT活性变化。Three yardlong bean cultivars were used as materials to carry out seeds germination experiments under 15 ℃ （low temperature）, 25℃ （control） and 35 ℃ （high temperature）, according to completely randomized design with 3 duplicates, respectively. To sample the radicles and cotyledons respectively while the radicles was 1 cm long, the total soluble protein （TSP）, the malondialdehyde （MDA） content, the activites of superoxide dismutase （SOD）, the catalase （CAT） and the peroxide enzyme （POD） were determined. The results indicated that MDA of both the radicles and cotyledons increased extremely significantly under the low and the high temperature treatments compared with the control, whereas the SOD activities decreased. TSP decreased in radicles either under low or under high temperature. As for cotyledons, TSP increased under low temperature, but decreased under high temperature. In cotyledons, the POD activities dropped down remarkably under the low temperature but increased under the high temperature, but the CAT activities increased significantly or extremely significantly under low temperature stress, but decreased under high temperature stress, whereas in radicles the changing tendencies were reverse. Therefore, most of indexes were influenced by temperature stresses, but the tendencies of influence by low and high temperature were not the same.

关 键 词：豇豆 温度胁迫 种子萌发生理 

分 类 号：S643.4[农业科学—蔬菜学]