胃炎和胃溃疡组织细胞的显微拉曼光谱研究  

Micro-Raman Spectra for Gastritis and Gastric Ulcer Tissues

作　　者：王慧敏[1] 张金彦[1] 郭建宇[1] 蔡炜颖[1] 孙真荣[1] 王祖赓[1] 房敏[2] 孙曼萍[2] 马淑颖[2] 

机构地区：[1]光谱学与波谱学教育部重点实验室,华东师范大学物理系,上海200062 [2]上海中医药大学附属岳阳中西医结合医院,上海200437

出　　处：《光谱学与光谱分析》2007年第10期2038-2041,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(10234030;10574046);国家重点基础研究项目(1999075204);教育部新世纪人才计划(NCET-04-0420);博士点基金(20050269011);上海科学技术委员会光科技(036105019);上海市教育委员会曙光学者计划(03SG23)资助

摘　　要：利用共焦显微拉曼光谱研究了胃炎组织和胃溃疡组织的粘膜细胞。结果表明,与胃炎组织细胞比较,溃疡组织细胞中胞嘧啶的振动峰781cm-1相对较弱,而腺嘌呤和胸腺嘧啶793,823cm-1处的峰强有所增加;对应于α螺旋结构蛋白质酰胺Ⅰ和酰胺Ⅲ带的1654和1230～1270cm-1的强度均相对减小,色氨酸特征峰1332cm-1和苯丙氨酸特征峰1003cm-1强度有所降低,同时与色氨酸残基微环境变化密切相关的1554频移到1556cm-1,且强度有所增加;脂类的特征谱线1073cm-1向高波数频移到了1078cm-1,对应于CH2扭转振动的1303cm-1与来自于CH面内变形振动的1268cm-1强度比值减小。上述变化表明两种疾病组织细胞中的核酸,蛋白质和脂类在成分、结构和构型上都存在明显差异;研究发现利用C—H弯曲振动的谱线1449cm-1和蛋白质酰胺Ⅲ带的谱线1660cm-1的强度比值A1449/A1660区分两种疾病具有较好的准确性。Micro-Raman spectroscopy was employed to identify gastritis tissues and gastric ulcer tissues. The primary spectral differences between the two types of samples include, for gastric ulcer tissues, （1） the intensity of the peak at 781 cm^-1 ascribed to cytosine decreases, while the peaks ascribed to adenine and thymine respectively at 793 and 823 cm^-1 become stmnger; （2） the hands of amide Ⅰ and amide Ⅲ at 1 654 and 1 320-1 270 cm^-1 respectively, characteristic of a-helix structural protein, lose their intensities, and the tryptophan band at 1 332 cm^-1 and phenylalanine band at 1 003 cm^-1 reduced significantly, while the tryptophan marker at 1 554 cm^-1 up shiftes to 1 556 cm^-1 with increasing intensity; （3） a blue shift of 1 073 cm^-1 line, the characteristic Raman hand of lipid, and a reduction in the ratio of 1 303 cm^-1 assigned to in-phase CH2 twisting motion to 1 268 cm^-1 from CH in-plane deformation were observed; （4）statistic analysis shows that the ratio of Raman intensities at bands 1 449 cm^-1 originating from CH2 group to 1 660 cm^-1 from amide Ⅰ provides a promising standard to distinguish the two tissues.

关 键 词：显微拉曼光谱 胃炎 胃溃疡 组织 

分 类 号：O657.3[理学—分析化学]