肺正常组织与癌变组织的表面增强拉曼光谱  

Surface-Enhanced Raman Spectra of Natural Tissue and Cancerous Tissue of Lung

作　　者：刘燕楠[1] 邹祖全[2] 刘燕青[3] 徐晓轩[1] 俞钢[1] 张存洲[1] 

机构地区：[1]南开大学物理科学学院光子学中心 [2]南开大学物理科学学院生物物理系 [3]武警医学院病理学教研室,天津300162

出　　处：《光谱学与光谱分析》2007年第10期2045-2048,共4页Spectroscopy and Spectral Analysis

基　　金：教育部"振兴计划"项目(A01504)资助

摘　　要：采用共焦显微拉曼光谱进行肺正常组织与癌变组织的表面增强拉曼光谱测试,拉曼位移测试范围为300～1700cm-1。根据得到的肺正常和癌变组织的表面增强拉曼光谱,对比分析了两者的特点与差异。选取蛋白质主链构象中构象不灵敏的CH2弯曲振动在1454cm-1的谱线为内标,发现相比肺正常组织,癌变组织蛋白质主链中酰胺Ⅲ和酰胺Ⅰ谱带的有序构象明显减少,对α螺旋和无规则卷曲的损伤比较严重,β回折几乎消失,而骨架C—N,C—C伸缩振动却出现了与前2个谱带截然相反的状况,有序构象显著增加,蛋白质侧链构象变化与主链相比则相对复杂;DNA的骨架磷酸基团、脱氧核糖、磷酸-脱氧核糖和碱基的含量总体呈增加趋势;RNA的情况与DNA类似;磷脂的链内纵向有序性参数明显下降。Surface-enhanced Raman spectra of natural tissue and cancerous tissue of lung from 300 to 1700 c^-1 were measured. In the cancerous tissue of lung, the orderly conformations of amides Ⅲ and amides Ⅰ of the main chains in protein such as α-helix, β-corner and no rules curly were damaged seriously; conversely, the extendable vibration of skeleton C--N and skeleton C--C increased. Side chains change became complicated. In general, the content of nucleic acids increases in the cancer tissue of lung. The lengthways conformations of lecithoid chains is out-of-order in the cancerous tissue of lung compared with the natural ones.

关 键 词：表面增强拉曼光谱 肺正常组织 肺癌变组织 

分 类 号：O657.3[理学—分析化学]