鉴别直肠组织癌变的环丙沙星铽络合物荧光染色新方法  

New Fluorescent Staining Method to Distinguish Normal and Malignant Rectum Tissues Using Terbium (Ⅲ)-Ciprofloxacin Complex

作　　者：孟涛[1] 徐端夫[1] 赵莹[1] 王笃金[1] 徐怡庄[2] 齐剑[2] 吴瑾光[2] 

机构地区：[1]中国科学院化学研究所高分子物理与化学国家重点实验室 [2]北京大学化学与分子工程学院,北京100871

出　　处：《光谱学与光谱分析》2007年第10期2069-2072,共4页Spectroscopy and Spectral Analysis

基　　金：国家重点基础研究发展规划项目(2002CCA01900);国家自然科学基金项目(30371604)资助

摘　　要：用具有较强荧光性能的环丙沙星铽配合物对多例直肠正常和直肠癌组织切片进行了染色研究,并与传统的HE染色结果进行了对比。结果表明用环丙沙星铽配合物染色后的效果与HE染色具有相同的图像和光学反差。并同时通过测定直肠正常和直肠癌组织的荧光光谱和红外光谱得到的组织病变判据,其结论与染色结果相一致。A new fluorescent staining method to distinguish normal and malignant rectum tissues using terbium（ Ⅲ ）-Ciprofloxacin complex was investigated. The terbium （ Ⅲ ） complex exhibits strong fluorescence and the staining process with the complex is very fast. It needs 3-5 minutes. The results showed that the fluorescence microscope images were similar to the images stained using traditional hematoxylin and eosin （H＆E） methodology. The fluorescence spectra and FTIR spectra of normal and malignant rectum tissues were collected and the detection criterions for the malignant rectum tissues were the same as the staining results. In summary, the fluorescence staining may be developed as a simple and convenient method.

关 键 词：铽络合物 环丙沙星 荧光染色 直肠癌 

分 类 号：O644.1[理学—物理化学]