不同水体中有色可溶性有机物质荧光光谱定量分析研究  

Determination of Chromphoric Dissolved Organic Matter in Water from Different Sources

作　　者：柳先平[1] 李磊[1] 戴金凤[1] 王小如[1] 黎先春[1] 

机构地区：[1]国家海洋局第一海洋研究所现代分析室,山东青岛266061

出　　处：《光谱学与光谱分析》2007年第10期2083-2087,共5页Spectroscopy and Spectral Analysis

基　　金：国家高技术研究发展计划"863"海洋污染物被动示踪检测技术项目(2003AA635180)资助

摘　　要：有色可溶性有机物(CDOM)是指水体中对紫外及可见光均有光吸收的溶解有机物,能对水色产生影响,是自然生态系统、水色光学遥感、水体碳循环研究中必不可少的环境因素。由于CDOM具有荧光特性,荧光光谱技术已成为开展CDOM研究的重要手段,被用于CDOM的来源、分布及其变化规律的研究。文章主要利用荧光检测技术,研究了不同水体CDOM的发射荧光光谱峰面积、荧光峰强度与其浓度间的变化关系,发现在低浓度范围内,发射光谱峰面积和荧光强度与CDOM浓度具有良好的线性关系(r2>0.98);不同浓度的CDOM水溶液的发射光谱峰面积及其荧光强度随浓度的变化趋势是一致的。文章首次利用低浓度下CDOM的发射光谱峰面积与浓度间的线性关系,建立了适合于水体中组成复杂的CDOM的定量分析方法,拓展了荧光分析技术在CDOM研究中的应用。Chromophoric dissolved organic matter （CDOM） represents the fraction of the dissolved organic pool which absorbs light in the visible as well as UV ranges. It could affect the color of the waters. It is necessary to study it during in research on ecosystem, remote sensing of the water color and the cycle of carbon in waters. CDOM can fluoresce when excited, so fluores- cence spectrum has been used to study its origin, distribution, and change. In the present article the fluorescence spectrophotometer was used to study the relation between the fluorescence intensity, spectrum area and the concentration of CDOM. When the concentration of CDOM is low（less than 75 mg · L^-1）, there is a better linear relationship（r^2〉0. 98）between the fluorescence intensity, the spectrum area and the concentration of CDOM. Meanwhile good linear relations were found between the fluorescence intensity and spectrum area, which showed the same changeable trend of the fluorescence intensity and spectrum area with the concentration change of CDOM. A method was established to quantify the concentration of CDOM in water from different source using the linear relationship between the spectrum area and the concentration. It suits the complicated constituent analysis of CDOM and could really and accurately show the concentration of CDOM in natural water.

关 键 词：有色可溶性有机物 定量分析 发射光谱 峰面积 

分 类 号：P733.3[天文地球—物理海洋学]