交替惩罚三线性分解算法结合三维荧光光谱法同时分辨和测定酪氨酸、苯丙氨酸和色氨酸  

Simultaneous Resolution and Determination of Tyrosine,Tryptophan and Phenylalanine by Alternating Penalty Trilinear Decomposition Algorithm Coupled with 3D Emission-Excitation Matrix Fluorometry

作　　者：肖劲[1] 任凤莲[1] 宋鸽[1] 廖律[1] 禹文峰[1] 曾涛[1] 

机构地区：[1]中南大学化学化工学院,现代仪器分析所,湖南长沙410083

出　　处：《光谱学与光谱分析》2007年第10期2088-2092,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(20475065)资助

摘　　要：用交替惩罚三线性分解算法(APTLD)结合三维荧光光谱法给出的二维数据对酪氨酸、苯丙氨酸和色氨酸进行了同时定性定量分析,为直接同时测定混合氨基酸中此三种物质提供了一种新的分析方法,其测定相关系数分别为0.9987,0.9995和0.9993。采用超声波组织细胞破碎法对木槿叶中的氨基酸进行提取,利用APTLD法对提取液中氨基酸进行定量测定,测定相对标准偏差分别为0.84%,0.36%,1.59%,回收率分别在101.0%～92.7%,106.5%～93.0%,103.0%～95.0%之间,方法简洁、快速、准确可靠,结果令人满意。A new method using alternating penalty trilinear decomposition algorithm coupled with excitation-emission matrix fluorometry has been developed for simultaneous resolution and determination of tyrosine, phenylalanine and tryptophan. Their correlation coefficients were 0. 998 7, 0. 999 5 and 0. 999 3 respectively. The contents of tyrosine, phenylalanine and tryptophan in Hibiscus syriacus L. leaves were also be determined by this method after being extracted by ultrasonic. The coefficients Of variation andthe recoveries of the three amino acids were 0. 84%, 0.36%, 1.59% and 101.0%-92. 7%, 106. 5%-93.0%, 103. 0%- 95.0% respectively. All these show that this is a simple, fast and cridible method.

关 键 词：交替惩罚三线性分解算法 三维荧光光谱 酪氨酸 色氨酸 苯丙氨酸 

分 类 号：O657.3[理学—分析化学]