毛细管胶束电动色谱法测定复方丹参滴丸中三种水溶性成份  

Separation and determination of three water-soluble components of compound danshen dripping pills by micellar electrokinetic chromatography

作　　者：乔成栋[1] 赵亮[1] 张红丽[1] 严祥[2] 宋平顺[3] 蒋生祥[1] 

机构地区：[1]中科院兰州化学物理研究所甘肃省天然药物重点实验室,兰州730000 [2]兰州大学第一医院,兰州730000 [3]甘肃省药品检验所,兰州730000

出　　处：《分析试验室》2007年第11期6-9,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(200675083);甘肃省自然科学基金(3ZS041-A25-23);中国科学院"西部之光"(2003)项目资助

摘　　要：在胶束电动色谱模式下考察了影响原儿茶醛、丹参素和原儿茶酸分离的重要因素并优化了关键的分离条件,建立了一种简单快速的利用毛细管胶束电动色谱DAD检测器分离分析原儿茶醛、丹参素、原儿茶酸的新方法。当缓冲溶液为50 mmol/L磷酸盐缓冲液(pH 7.2)和20 mmol/L SDS,分离电压为15 kV和分离温度为25℃时,三种分析物在10 min内实现了基线分离。结果表明,所建立的分离方法的线性范围宽,检出限低,重现性好。并利用该方法测定了复方丹参滴丸中的原儿茶醛和丹参素。Based on the investigation of some key factors, applied to separate Protocatechuic Aldehyde, Danshensu andProtocatechuic Acid, a novel, simple and effective method for the separation and determination by DAD detector with miceUar electrokinetic chromatography has been developed. Their baseline separation is achieved with satisfactory repeatability and high separation efficiency in 10 minutes using 50 mmol/L phosphate buffer （pH 7.2） containing 20 mmol/L SDS, under 15 kV applied voltage, and at the temperature of 25 ℃. The results show that the method turns out to be quite satisfactory with the wide linear range, the low detection limit and good repeatability. In addition, this method is adopted to determine the contents of Protocatechuic Aldehyde and Danshensu in Compound Danshen Dripping Pills.

关 键 词：胶束电动色谱 复方丹参滴丸 原儿茶醛 丹参素 原儿茶酸 

分 类 号：O557.7[理学—热学与物质分子运动论]