猪血中血红素提取条件的比较  

Comparison of the extraction conditions for heme from pig blood

作　　者：王颖明[1] 莫宝庆[1] 徐济达[1] 李庆天[1] 

机构地区：[1]南京医科大学公共卫生学院,南京210029

出　　处：《食品科技》2007年第10期246-247,254,共3页Food Science and Technology

基　　金：江苏省卫生厅科研基金项目(H9505)。

摘　　要：目的:选择由猪血中提取血红素的最佳条件。方法:采用新鲜猪血抗凝离心后取血球,水浴加热溶血后,用3%盐酸丙酮提取血红素,提取液加鞣酸/氯化锶沉淀分离出血红素。结果:溶血时血球与水的比例以1∶1,抽提时用5倍于血球体积的丙酮,过滤时用细纱布过滤,提取效率较高,而沉淀血红素时加鞣酸或加氯化锶均可,加入量以5g/100mL为佳。结论:提取条件对血红素提取效率有较大影响。Objectives： To select the optimal conditions for the extraction of heme from pig blood. Methods： Red blood cell （RBC） was isolated from the anti-coagulated fresh pig blood, It was hemolyzed by heated water, then heme was extracted with 3% acetone-hydrochloric acid. Then heme was obtained from the extraction with the help of tannin or strontium chloride. Results： The heme was extracted more when the ratio of RBC to water was 1：1 in the hemolysis, the ratio of RBC to acetone was 1：5 in extraction, tiny gauze was used to deposit the heme during filtration. The effect of tannin or strontium chloride was similar in the deposition of heme, the optimal concentration was 5g/100mL. Conclusion： Heme extraction is greatly influenced by the extraction conditions.

关 键 词：鲜猪血 血红素 提取 条件 

分 类 号：TS251.9[轻工技术与工程—农产品加工及贮藏工程]