Visualization and Characterization of High-Order Chromatin Fibers under Light Microscope during Interphase and Mitotic Stages in Plants  

作　　者：Jing-Yu Liu Chao-Wen She Zhong-Li Hu Fen Li Ying Diao Li-Hua Liu Yun-Chun Song 

机构地区：[1]Human Genome Research Center and College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074 China [2]Key Laboratory of the Ministry of Education of Plant Developmental Biology, Wuhan University, Wuhan 430072, China [3]Department of Biology, Huaihua College, Huaihua 418008, China

出　　处：《Journal of Integrative Plant Biology》2007年第11期1634-1639,共6页植物学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(39870423 and 30670736);China Postdoctoral Science Foundation(2003034496).

摘　　要：Using genomic in situ hybridization with genomic DNA, high-order chromatin fibers were successfully exhibited under a light microscope through the cell cycle in barley, rice, maize and field bean. From the interphase to prophase and metaphase of mitosis, the fibers were basically similar. Each was estimated to be around 200 nm in diameter, but the strength of signals was not the same along the fiber length. Through the cell cycle a series of dynamic distribution changes occurred in the fibers. In the interphase, they were unraveled. At the early prophase they were arranged with parallel and mirror symmetry. During late-prophase and metaphase, the fibers were bundled and became different visible chromosomes. The parallel coiling and mirror symmetry structures were visible clearly until the metaphase. In anaphase they disappeared. During telophase, in peripheral regions of congregated chromosome group, borderlines of the chromosomes disappeared and the fibers were unraveled. This demonstrated that mitotic chromosomes are assembled and organized by parallel and adjacent coiling of the fibers and the fibers should be the highest order structure for DNA coiling.Using genomic in situ hybridization with genomic DNA, high-order chromatin fibers were successfully exhibited under a light microscope through the cell cycle in barley, rice, maize and field bean. From the interphase to prophase and metaphase of mitosis, the fibers were basically similar. Each was estimated to be around 200 nm in diameter, but the strength of signals was not the same along the fiber length. Through the cell cycle a series of dynamic distribution changes occurred in the fibers. In the interphase, they were unraveled. At the early prophase they were arranged with parallel and mirror symmetry. During late-prophase and metaphase, the fibers were bundled and became different visible chromosomes. The parallel coiling and mirror symmetry structures were visible clearly until the metaphase. In anaphase they disappeared. During telophase, in peripheral regions of congregated chromosome group, borderlines of the chromosomes disappeared and the fibers were unraveled. This demonstrated that mitotic chromosomes are assembled and organized by parallel and adjacent coiling of the fibers and the fibers should be the highest order structure for DNA coiling.

关 键 词：high-order chromatin fibers INTERPHASE mitosis. 

分 类 号：Q94[生物学—植物学]