限制性内切酶介导的串珠镰刀菌插入突变和致病性突变体的分离  

Inserter mutation by restriction enzyme-mediated integration in Fusarium moniliforme and isolation of pathogenicity mutant

作　　者：赵培宝[1] 周庆新[1] 郭芳先[1] 李多川[1] 

机构地区：[1]山东农业大学植物保护学院

出　　处：《植物病理学报》2007年第5期545-548,共4页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(30571246)

摘　　要：For studying on pathogenicity mechanism of Fusarium moniliforme,REMI was used to transform protoplasts of FT1 strain with the vector pUCATPH,which contained hygromycin B-resistant gene.More than 300 transformants had been obtained,most of them were quite stable after five rounds of successive culture.25 mutants of morphology and 2 weak pathogenicity mutants were gained by REMI.PCR amplification showed that the hygromycin B-resistant gene had integrated into genomes of the two pathogenicity mutants.The optimum conditions of preparing protoplasts were: the mycelia growing in PDB medium for 14 h,lywallzyme was used to digest the mycelia at 100 r/min,30℃ for 4 h,and 0.7 mol/L NaCl was used as the osmotic stabilizer.For studying on pathogenicity mechanism of Fusarium moniliforme, REMI was used to transform protoplasts of ET1 strain with the vector pUCATPH, which contained hygromycin B-resistant gene. More than 300 transformants had been obtained, most of them were quite stable after five rounds of successive culture. 25 mutants of morphology and 2 weak pathogenicity mutants were gained by REMI. PCR amplification showed that the hygromycin B-resistant gene had integrated into genomes of the two pathogenicity mutants. The optimum conditions of preparing protoplasts were ： the mycelia growing in PDB medium for 14 h, lywallzyme was used to digest the mycelia at 100 r/min, 30℃ for 4 h, and 0.7 mol/L NaCl was used as the osmotic stabilizer.

关 键 词：插入突变 限制性内切酶 突变体 串珠镰刀菌 分离 致病性 生物基因组 遗传转化 

分 类 号：S828.2[农业科学—畜牧学]