利用多重RT-PCR技术检测草莓病毒的研究  

Detection of strawberry viruses by multiplex RT-PCR

作　　者：杨洪一[1] 张志宏[2] 李丽丽[2] 赵敏[1] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040 [2]沈阳农业大学园艺学院,沈阳110161

出　　处：《植物病理学报》2007年第5期549-552,共4页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(30200187);黑龙江省博士后基金资助项目

摘　　要：Coat protein gene of 12 isolates of Strawberry vein banding virus(SVBV) was studied by multiple sequence alignment and the primers located in conserved region were designed.The detection protocol for SVBV by reverse transcriptase polymerase chain reaction(RT-PCR) was developed.The primers of multiplex RT-PCR were selected by primer-primer interactions and the melting temperature.The annealing temperature,the concentration of PCR buffer,the extension temperature,the extension time and the concentration of pri-mers were optimized,respectively.A multiplex RT-PCR assay was made for simultaneous detecting Strawberry mottle virus(SMoV),Strawberry mild yellow edge virus(SMYEV) and SVBV.Both field-grown strawberries and microplants were detected effectively.It was the first report that multiplex RT-PCR was used to assay the efficacy of strawberry viruses elimination.Coat protein gene of 12 isolates of Strawberry vein banding virus （SVBV） was studied by multiple sequence alignment and the primers located in conserved region were designed. The detection protocol for SVBV by reverse transcriptase polymerase chain reaction （RT-PCR） was developed. The primers of multiplex RT-PCR were selected by primer-primer interactions and the melting temperature. The annealing temperature, the concentration of PCR buffer, the extension temperature, the extension time and the concentration of primers were optimized, respectively. A multiplex RT-PCR assay was made for simultaneous detecting Strawberry mottle virus （SMoV）, Strawberry mild yellow edge virus （SMYEV） and SVBV. Both field-grown strawberries and microplants were detected effectively. It was the first report that multiplex RT-PCR was used to assay the efficacy of strawberry viruses elimination.

关 键 词：草莓镶脉病毒 PCR技术 斑驳病毒 技术检测 virus RT 多重 混合侵染 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]