猪ZAR1基因变异及其对产仔数的影响  

Mutations of Porcine Zygote Arrest 1(ZAR1)Gene and Its Effects on Litter Size

作　　者：高建[1] 王振骅 龚炎长[1] 杨利国[1] 彭秀丽[1] 刘俊[1] 柳友茂 袁金锋[1] 刘辉[1] 胡修忠[1] 李文明[1] 刘洪彬[1] 戴霞亮 王前刚 张淑君[1] 

机构地区：[1]华中农业大学农业动物遗传育种与繁殖教育部重点实验室,武汉430070 [2]湖北天种畜牧股份有限公司,黄陂430344

出　　处：《农业生物技术学报》2007年第5期735-740,共6页Journal of Agricultural Biotechnology

基　　金：国家农业科技转化基金(No.04EFN214200206);武汉市重大攻关项目(No.20022002061);武汉市国际合作项目(No.20067003111-06);湖北省国际合作项目(No.2003AA211041)资助

摘　　要：根据合子阻滞因子1(zygotearrest 1,ZAR1)基因GenBank的DNA序列(DQ231443,gi:83727928)设计2对引物,用直接测序和PCR-SSCP相结合的方法,进行单核苷酸多态性(single nucleotide polymorphism,SNP)检测,分析该基因在5个品种猪(小梅山、清平、杜洛克、长白和大白)中变异特征及其与产仔数的相关性。测序后在内含子2、3和外显子3中存在6个SNP及在内含子3中存在1处5bp的插入/缺失变异。在5个品种猪群中,对外显子3中的第54碱基处C→T突变(Exon3-BstUⅠ)和内含子3中的5个碱基插入/缺失的变异(Intron3-TspRⅠ)进行了PCR-RFLP检测,并与产仔数进行了关联分析,结果表明,Exon3-BstUⅠ多态对杜洛克母猪第1胎和2胎总产仔数产生显著(P<0.05)影响,对经产母猪总产仔数产生极显著(P<0.01)影响,对经产母猪的产活仔数影响极显著(P<0.01),携带CC基因型母猪的产仔数均高于TT基因型的;Intron3-TspRⅠ多态对杜洛克母猪第1胎和经产胎次总产仔数和产活仔数有显著(P<0.05)影响,NN基因型高于MN和MM基因型的产仔数。The zygote arrest 1 （ZAR1） gene （gi：83727928） was employed to investigate variation character and associate with reproductive traits in five pig purebreds （Small Meishan, Qingping, Duroc, Landrace and Large White）. Six new single nucleotide polymorphisms （SNPs） in the second and third exons, addition with one 5 bp deletion/insertion mutation in the third intron in the ZAR1 gene were identified by using PCR-SSCP and DNA sequencing technique. Exon 3-BstU Ⅰ（C^54/T^54） located in the 54th of Exon 3 and Intron 3-TspR Ⅰ（5 bp deletion/insertion mutation） in intron 3 were genotyped and associated with reproductive traits in these five pig breeds. Association results showed that Exon 3-BstU Ⅰ polymorphism had a significant effective （P 〈0.05） with total number born in first litter （TNB^1） and total number born in second litter（TNB^2）. Total number of born alive （NBA） with CC genotype had more piglets than that of other genotypes in the Exon 3-BstU Ⅰ in the Duroc. Intron 3-TspR Ⅰ polymorphism had a significant effective （P〈0.05） with total number born in first litter （TNB^1） and in later litters （TNB^2） in the Duroc.

关 键 词：猪 产仔数 合子阻滞因子 单核苷酸多态性 

分 类 号：S188[农业科学—农业基础科学]