用水稻基因芯片筛选小麦耐旱相关基因  

Drought-tolerant Gene Screening in Wheat by Using Rice Microarray

作　　者：许州达[1] 景瑞莲[2] 甘强[3] 曾海攀[4] 孙学辉[2] Leung Hei 路铁刚[2] 刘国振[1] 

机构地区：[1]河北农业大学生命科学学院,保定071000 [2]中国农业科学院,北京100081 [3]中国科学院遗传发育研究所,北京100101 [4]中国科学院北京基因组研究所,北京101300 [5]国际水稻研究所

出　　处：《农业生物技术学报》2007年第5期821-827,共7页Journal of Agricultural Biotechnology

基　　金：菲律宾国际水稻研究所Generation Challenge Program(GCP);国家重点基础研究发展规划(973)项目(No.2006CB100202)资助

摘　　要：为了解小麦在干旱逆境条件下的基因转录规律,采用PEG6000对耐旱小麦(Triticum aestivum)品种旱选10号进行拟旱处理,分别提取0、1、6和24h植株的总RNA,经反转录荧光标记制备cDNA探针,并将其与含有6万Oligo的水稻全基因组芯片进行杂交,扫描采集数据后并进行结果分析。在1、6和24h样品中分别检测到差异表达基因166、207和328个,随着处理时间的延长,差异表达基因数目增加。对差异基因进行功能分类,能量代谢途径相关基因在1、6和24h差异表达基因总数中所占比例分别为4.2%、8.2%和16.8%,其中大部分为光合作用相关基因,并且主要表现为上调,但Psbr和Rubisco编码基因的转录水平为下调,暗示它们在耐旱反应中发挥着一定作用。To investigate the wheat transcriptional profiling under drought stress, a drought tolerance wheat （Triticum aestivum） variety Hanxuan 10 was treated by PEG6000 and the total RNA samples were collected at 0, 1, 6, and 24 h, respectively. Complementary DNA were labeled with florescence dye and used to hybridize with the BGI-Rice Chip, a whole genome rice gene chip platform, which contains over 60 000 Oligos designed based on rice genome sequence. Data analysis detected 166, 207 and 328 differentially expressed genes （DGs） at 1, 6 and 24 h, respectively, indicated that the number of DGs increased along with PEG treatment. Functional category analysis found that the number of DGs related to energy metabolism pathway increased and their proportion to the total number of DGs were 4.2%, 8.2% and 16.8%, respectively. Most of the photosynthesis-related genes were up-regnlated. It is interesting to note that Psbrand Rubisco-coding genes were down-regnlated, suggesting their potential role in drought tolerance response.

关 键 词：小麦 耐旱 基因芯片 转录 

分 类 号：S188[农业科学—农业基础科学]