湖南典型茶树地理种群遗传多样性  

RAPD Analysis for Genetic Diversity of Typical Tea Populations in Hunan Province

作　　者：沈程文[1] 黄意欢[1] 黄建安[1] 罗军武[1] 刘春林[1] 刘德华[1] 

机构地区：[1]湖南农业大学园艺园林学院教育部茶学重点实验室作物基因工程湖南省重点实验室,长沙410128

出　　处：《农业生物技术学报》2007年第5期855-860,共6页Journal of Agricultural Biotechnology

基　　金：湖南省自然科学基金项目(No.OOJJY20107)资助

摘　　要：利用RAPD分子标记技术,对湖南典型茶树安化云台山种(Camellia sinensis var.sinensis)、城步峒茶(C.sinensis var.assamica cv.Duntsa)、汝城白毛茶(C.ptilophylla)和江华苦茶(C.sinensis var.assamica cv.Jianghua)4个地理种群的240个单株的遗传多样性、种群内和种群间的遗传变异进行了研究。结果表明,21个10碱基随机引物共检测到226条谱带,其中多态性谱带为201条,占88.9%。遗传多样性分析结果显示:Shannon's多样性指数为0.43,种群内变异占74.7%,而种群间变异占25.3%;Nei's指数群体总基因多样性(HT)为0.37,群体内平均基因多样性(HS)为0.28,群体间的基因多样性(HST)为0.09,群体Nei's基因分化系数(GST)为0.23,说明76.7%的变异存在于种群间,群体内的变异占了总变异的23.3%,与Shannon's多样性指数相比基本一致,均表明种群内有较丰富的遗传变异;种群间的基因流(Nm)为0.74,显示种群间的基因交流有限。Genetic diversity and genetic variation of 240 adult plants of four tea populations, Camellia sinensis var. sinensis, C. sinensis vat. assamica cv. Duntsa, C. ptilophylla and C. sinensis var. assamica cv. Jianghua in Hunan, were studied by using RAPD markers. The results showed that 226 loci were found by using 21 random primers （10 bp）, of which 201 loci were polymorphic （88.9%）. The analyzing result of genetic diversity indicated that Shannon＇s index was 0.43, 74.7% of which was within population genetic diversity while 25.3% was among population variation; the gene diversity of total populations （HT） of Nei＇s index was 0.37, gene diversity within populations （Hs） was 0.28, gene diversity among populations （HST） was 0.09 and the coefficient of gene differentiation （GST） among the populations was 0.23, indicating that 76.7% of variation was within populations while 23.3% was among populations, which showed that there was rich genetic variation within population variation, just the same as Shannon＇s diversity index. Gene flow （Nm） from inter-population was 0.74, which indicated the limited genetic exchange between populations.

关 键 词：茶树 RAPD分析 种群 遗传多样性 

分 类 号：S188[农业科学—农业基础科学]